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CHSY3 rabbit polyclonal antibo

dy, Aff - Purified
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  • ¥1999
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  • AP01393PU-N
  • 2026年03月07日
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      100 ug

    gene_symbol:CHSY3
    predicted_size:~ 85 kDa
    Uniprot ID:Q7JA7
    description:CHSY3 rabbit polyclonal antibody, Aff - Purified
    clonality:Polyclonal
    product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...):Primary Antibodies
    background:Chondroitin sulfate synthases (CHSYs) synthesize chondroitin sulfate, a glycosaminoglycan expressed on the surface of most cells and in extracellular matrices. Glycosaminoglycan chains are covalently linked to various of core protein families and regulate many biologic processes, including extracellular matrix deposition, cell proliferation and recognition, and morphogenesis. The CHSY family includes CHSY1, CHSY2 and CHSY3. CHSY1 and CHSY3 display both glucuronyltransferase and N-acetylgalactosaminyltransferase activities, while CHSY2 is required for chondroitin polymerizing activity. CHSY2 localizes to the Golgi apparatus and is expressed ubiquitously, with highest expression observed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, small intestine and placenta.
    immunogen:Synthetic peptide, corresponding to amino acids 640-690 of Human CHSY2.
    recommended_dilution: Western Blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200. Immunofluorescence: 1/50-1/200.
    predicted_size:~ 85 kDa
    buffer:PBS, approx. pH 7.2; State: Aff - Purified; State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE); Preservative: 15 mM Sodium Azide
    host_species:Rabbit
    applications:IF, IHC, WB
    reactivities:Human, Mouse, Rat

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    相关实验
    • Use of EIA to Measure MMPs and TIMPs

      the combination of a monoclonal antibody as the solid-phase with rabbit polyclonal antibody and horseradish peroxidase (HRP)-labeled donkey antirabbit gamma globulin as the second phase (2 ). EIAs for human MMP-9 were developed using MMP-9 specific polyclonal

    • Tips and hints for the storage of antibodies

      ! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses. Rabbit antisera Antisera are more robust than monoclonals. With anti-microbials added, they may be stored at  ℃. Serum does not contain active proteases

    • Immunocytology using the HA epitope

      . A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.

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