Mouse/Human/Rat Phospho-LMNA /
Lamin A+C (Ser22) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1751- $740
- LSBio
- To be determined
- LS-F1751
- 美国
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse, Human, Rat
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
To be determined
- 供应商：
康朗生物
- 规格：
96T
Mouse/Human/Rat Phospho-LMNA / Lamin A+C (Ser22) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1751
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Product Description
LS-F1751 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human LMNA / Lamin A+C in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.Specifications
TypeCell-Based Phoshorylation ELISA (enzyme-linked immunosorbent assay) kit
Target
LMNA / Lamin A+C
Reactivity
Mouse, Human, Rat
Specificity
Lamin A/C (Phospho-Ser22)
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Cell Culture Microplate
- Blocking Buffer
- Detection Antibodies
- HRP-Conjugate
- Substrate
- Stop Solution
- Crystal Violet Solution
- SDS Solution
- Adhesive Plate Sealers
Protocol
- Seed cells into wells and incubate overnight at 37°C, 5% CO2.
- Apply desired treatment conditions.
- Add 100 µl of Fixing Solution and incubate 20 minutes at room temperature.
- Add 100 µl of Quenching Buffer and incubate 20 minutes at room temperature.
- Add 200 µl of Blocking Buffer and incubate for 1 hour at room temperature.
- Add 50 µl of 1x primary antibodies and incubate overnight at 4°C. Add 50 µl of HRP-conjugated secondary antibodies and incubate for 1.5 hours at room temperature.
- Add 50 µl of Ready-to-Use Substrate and incubate for 30 minutes at room temperature.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About LMNA / Lamin A+C
P02545 NM_005572 NP_005563.1
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Cell-Based Phosphorylation ELISA Platform Overview
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文献和实验A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
的关系理不出头绪。GSK-3 beta 总蛋白减少，p-GSK-3 beta (ser 9)增加，是不是足以说明有活性的GSK-3 beta 减少更多，这样理解不知道对不对，希望给以指点。 galaxy22 楼主你好，请问你做出结果的tyr216的抗体是哪个公司的。。。我做出来总量是减少的，active B catenin是减少的，那我有必要做ser9 和tyr216的磷酸化形式吗？我想研究wnt和akt。。。 棉花糖的温柔
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
in this protocol. Collect suspended cells and place on ice until use. Determine the cell number by using a hemacytometer or other cell counter. 2. Preheat the water bath to 65°C 3. Water bath or heating block preset at 37°C 4. Pre-set
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