pCXUN植物表达质粒

基本信息

启动子:	Ubi promoter
终止子:	NOS terminator
质粒大小:	12008bp
原核抗性:	卡那霉素Kan
筛选标记:	潮霉素Hyg
克隆菌株:	大肠杆菌DB3.1
培养条件:	37℃，有氧，LB

质粒简介

        pCXUN质粒是一个植物过表达载体，Ubi启动子驱动目的基因的表达。使用XcmI酶切空载体得到T载体，可连入两端带A的PCR产物，并在ccdb致死基因的帮助下，基因克隆的阳性率得以大大提高。

质粒图谱

质粒序列

LOCUS       Exported               12008 bp ds-DNA     circular SYN 30-OCT-2017
DEFINITION  synthetic circular DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12008)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12008
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      115..367
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(439..744)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     promoter        complement(1101..3091)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_feature    3332..3356
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4655..5284
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5718..6785
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6851..7045
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7389..7529
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7715..8303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8390..9184)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9609..9633
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9711..9885
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9925..10950)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(11017..11694)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 gaattcgagc tcggtacccc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
       61 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcccgatcta
      121 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
      181 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
      241 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
      301 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
      361 aacgatcggg gaaattcgga tccccaatac ttgtatgggt cgacctgcag actggctgtg
      421 tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc
      481 attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc
      541 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc
      601 acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc
      661 gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt
      721 ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt
      781 tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt
      841 cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga
      901 catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg
      961 cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata tattcttata
     1021 ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccccataca
     1081 agtattgggg atcccccggg ctgcagaagt aacaccaaac aacagggtga gcatcgacaa
     1141 aagaaacagt accaagcaaa taaatagcgt atgaaggcag ggctaaaaaa atccacatat
     1201 agctgctgca tatgccatca tccaagtata tcaagatcaa aataattata aaacatactt
     1261 gtttattata atagataggt actcaaggtt agagcatatg aatagatgct gcatatgcca
     1321 tcatgtatat gcatcagtaa aacccacatc aacatgtata cctatcctag atcgatattt
     1381 ccatccatct taaactcgta actatgaaga tgtatgacac acacatacag ttccaaaatt
     1441 aataaataca ccaggtagtt tgaaacagta ttctactccg atctagaacg aatgaacgac
     1501 cgcccaacca caccacatca tcacaaccaa gcgaacaaaa agcatctctg tatatgcatc
     1561 agtaaaaccc gcatcaacat gtatacctat cctagatcga tatttccatc catcatcttc
     1621 aattcgtaac tatgaatatg tatggcacac acatacagat ccaaaattaa taaatccacc
     1681 aggtagtttg aaacagaatt ctactccgat ctagaacgac cgcccaacca gaccacatca
     1741 tcacaaccaa gacaaaaaaa agcatgaaaa gatgacccga caaacaagtg cacggcatat
     1801 attgaaataa aggaaaaggg caaaccaaac cctatgcaac gaaacaaaaa aaatcatgaa
     1861 atcgatcccg tctgcggaac ggctagagcc atcccaggat tccccaaaga gaaacactgg
     1921 caagttagca atcagaacgt gtctgacgta caggtcgcat ccgtgtacga acgctagcag
     1981 cacggatcta acacaaacac ggatctaaca caaacatgaa cagaagtaga actaccgggc
     2041 cctaaccatg gaccggaacg ccgatctaga gaaggtagag aggggggggg ggggaggacg
     2101 agcggcgtac cttgaagcgg aggtgccgac gggtggattt gggggagatc tggttgtgtg
     2161 tgtgtgcgct ccgaacaaca cgaggttggg gaaagagggt gtggaggggg tgtctattta
     2221 ttacggcggg cgaggaaggg aaagcgaagg agcggtggga aaggaatccc ccgtagctgc
     2281 cgtgccgtga gaggaggagg aggccgcctg ccgtgccggc tcacgtctgc cgctccgcca
     2341 cgcatttctg gatgccgaca gcggagcaag tccaacggtg gagcggaact ctcgagaggg
     2401 gtccagaggc agcgacagag atgccgtgcc gtctgcttcg cttggcccga cgcgacgctg
     2461 ctggttcgct ggttggtgtc cgttagactc gtcgacggcg tttaacaggc tggcattatc
     2521 tactcgaaac aagaaaaatg tttccttagt ttttttaatt tcttaaaggg tatttgttta
     2581 atttttagtc actttatttt attctatttt atatctaaat tattaaataa aaaaactaaa
     2641 atagagtttt agttttctta atttagaggc taaaatagaa taaaatagat gtactaaaaa
     2701 aattagtcta taaaaaccat taaccctaaa ccctaaatgg atgtactaat aaaatggatg
     2761 aagtattata taggtgaagc tatttgcaaa aaaaaaggag aacacatgca cactaaaaag
     2821 ataaaactgt agagtcctgt tgtcaaaata ctcaattgtc ctttagacca tgtctaactg
     2881 ttcatttata tgattctcta aaacactgat attattgtag tactatagat tatattattc
     2941 gtagagtaaa gtttaaatat atgtataaag atagataaac tgcacttcaa acaagtgtga
     3001 caaaaaaaat atgtggtaat tttttataac ttagacatgc aatgctcatt atctctagag
     3061 aggggcacga ccgggtcacg ctgcactgca ggaattcgat atcaagcttg gcactggccg
     3121 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     3181 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     3241 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg
     3301 tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     3361 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt
     3421 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat
     3481 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
     3541 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
     3601 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
     3661 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
     3721 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
     3781 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
     3841 ccacctagcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
     3901 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
     3961 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
     4021 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
     4081 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
     4141 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
     4201 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
     4261 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
     4321 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
     4381 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
     4441 agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
     4501 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
     4561 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
     4621 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
     4681 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
     4741 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
     4801 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
     4861 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
     4921 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
     4981 ttacgacata tgggcaaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
     5041 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
     5101 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
     5161 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
     5221 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
     5281 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
     5341 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
     5401 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
     5461 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
     5521 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
     5581 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
     5641 gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc
     5701 cgaggaatcg gcgtgacggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     5761 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
     5821 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     5881 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     5941 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     6001 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     6061 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
     6121 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     6181 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     6241 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     6301 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     6361 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     6421 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
     6481 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     6541 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     6601 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     6661 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     6721 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
     6781 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
     6841 gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
     6901 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
     6961 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
     7021 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
     7081 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
     7141 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
     7201 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
     7261 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     7321 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     7381 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     7441 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     7501 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     7561 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7621 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7681 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7741 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7801 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7861 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7921 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7981 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8041 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8101 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8161 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8221 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8281 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8341 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt
     8401 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
     8461 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
     8521 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
     8581 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
     8641 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
     8701 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
     8761 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
     8821 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
     8881 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
     8941 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
     9001 ccttttcccg ttcaacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
     9061 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
     9121 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
     9181 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
     9241 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
     9301 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
     9361 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
     9421 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
     9481 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
     9541 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
     9601 ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac
     9661 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt
     9721 ttagtactgg attttggttt taggaattag aaattttatt gatagaagta ttttacaaat
     9781 acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc
     9841 ctaattccct tatctgggaa ctactcacac attattatgg agaaactcga gcttgtcgat
     9901 cgacagatcc ggtcggcatc tactctattt ctttgccctc ggacgagtgc tggggcgtcg
     9961 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
    10021 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
    10081 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
    10141 accaatgcgg agcatatacg cccggagtcg tggcgatcct gcaagctccg gatgcctccg
    10201 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
    10261 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat
    10321 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
    10381 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
    10441 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
    10501 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
    10561 cgtctggcta agatcggccg cagcgatcgc atccatagcc tccgcgaccg gttgtagaac
    10621 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat
    10681 gcaataggtc aggctctcgc taaactcccc aatgtcaagc acttccggaa tcgggagcgc
    10741 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
    10801 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
    10861 ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac
    10921 agacgtcgcg gtgagttcag gctttttcat atctcattgc cccccggatc tgcgaaagct
    10981 cgagagagat agatttgtag agagagactg gtgatttcag cgtgtcctct ccaaatgaaa
    11041 tgaacttcct tatatagagg aaggtcttgc gaaggatagt gggattgtgc gtcatccctt
    11101 acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
    11161 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
    11221 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt
    11281 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc
    11341 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg
    11401 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tatcacatca atccacttgc
    11461 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
    11521 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
    11581 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
    11641 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
    11701 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
    11761 accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg cgttggccga
    11821 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    11881 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    11941 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    12001 atgattac