pMD2.G慢病毒包装质粒

基本信息

启动子:	CMV
复制子:	pUC ori
终止子:	beta-globin poly(A)
质粒分类:	病毒系列，慢病毒包装载体
质粒大小:	5822bp
质粒标签:	VSV-G
原核抗性:	Amp
克隆菌株:	Stbl3
培养条件:	37℃，有氧 LB
表达宿主:	哺乳细胞
诱导方式:	无须诱导，瞬时表达
5'测序引物:	CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物:	根据序列设计引物

质粒简介

         PMD2. belong to the VSV-G expression plasmid, and can be used together with pSPAX2.

质粒图谱

质粒序列

LOCUS       Exported                5822 bp ds-DNA     circular SYN 24-OCT-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, October 24, 2016 from SnapGene Viewer 3.2.1
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_site      1048..1442
                     /note="beta-globin poly(A)"
                     /note="human beta-globin polyadenylation signal"
     rep_origin      complement(1952..2540)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2711..3571)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3572..3676)
                     /gene="bla"
                     /note="AmpR promoter"
     enhancer        3867..4246
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4247..4450
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          4584..5059
                     /note="beta-globin intron"
                     /note="internally truncated intron from human beta-globin"
     CDS             5134..847
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /note="VSV-G"
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
ORIGIN
        1 tggaggcaag gcctgcaaaa tgcaatactg caagcattgg ggagtcagac tcccatcagg
       61 tgtctggttc gagatggctg ataaggatct ctttgctgca gccagattcc ctgaatgccc
      121 agaagggtca agtatctctg ctccatctca gacctcagtg gatgtaagtc taattcagga
      181 cgttgagagg atcttggatt attccctctg ccaagaaacc tggagcaaaa tcagagcggg
      241 tcttccaatc tctccagtgg atctcagcta tcttgctcct aaaaacccag gaaccggtcc
      301 tgctttcacc ataatcaatg gtaccctaaa atactttgag accagataca tcagagtcga
      361 tattgctgct ccaatcctct caagaatggt cggaatgatc agtggaacta ccacagaaag
      421 ggaactgtgg gatgactggg caccatatga agacgtggaa attggaccca atggagttct
      481 gaggaccagt tcaggatata agtttccttt atacatgatt ggacatggta tgttggactc
      541 cgatcttcat cttagctcaa aggctcaggt gttcgaacat cctcacattc aagacgctgc
      601 ttcgcaactt cctgatgatg agagtttatt ttttggtgat actgggctat ccaaaaatcc
      661 aatcgagctt gtagaaggtt ggttcagtag ttggaaaagc tctattgcct cttttttctt
      721 tatcataggg ttaatcattg gactattctt ggttctccga gttggtatcc atctttgcat
      781 taaattaaag cacaccaaga aaagacagat ttatacagac atagagatga accgacttgg
      841 aaagtaactc aaatcctgca caacagattc ttcatgtttg gaccaaatca acttgtgata
      901 ccatgctcaa agaggcctca attatatttg agtttttaat ttttatgaaa aaaaaaaaaa
      961 aaaacggaat tcaccccacc agtgcaggct gcctatcaga aagtggtggc tggtgtggct
     1021 aatgccctgg cccacaagta tcactaagct cgctttcttg ctgtccaatt tctattaaag
     1081 gttcctttgt tccctaagtc caactactaa actgggggat attatgaagg gccttgagca
     1141 tctggattct gcctaataaa aaacatttat tttcattgca atgatgtatt taaattattt
     1201 ctgaatattt tactaaaaag ggaatgtggg aggtcagtgc atttaaaaca taaagaaatg
     1261 aagagctagt tcaaaccttg ggaaaataca ctatatctta aactccatga aagaaggtga
     1321 ggctgcaaac agctaatgca cattggcaac agcccctgat gcctatgcct tattcatccc
     1381 tcagaaaagg attcaagtag aggcttgatt tggaggttaa agttttgcta tgctgtattt
     1441 tacattactt attgttttag ctgtcctcat gaatgtcttt tcactaccca tttgcttatc
     1501 ctgcatctct cagccttgac tccactcagt tctcttgctt agagatacca cctttcccct
     1561 gaagtgttcc ttccatgttt tacggcgaga tggtttctcc tcgcctggcc actcagcctt
     1621 agttgtctct gttgtcttat agaggtctac ttgaagaagg aaaaacaggg ggcatggttt
     1681 gactgtcctg tgagcccttc ttccctgcct cccccactca cagtgacccg gaatccctcg
     1741 acatggcagt ctagcactag tgcggccgca gatctgcttc ctcgctcact gactcgctgc
     1801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2581 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2641 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2701 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2761 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2821 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2881 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2941 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3001 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3061 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3121 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3181 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3241 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3301 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3361 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3421 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3481 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3541 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3601 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3661 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtggat cccctgaggg
     3721 ggcccccatg ggctagagga tccggcctcg gcctctgcat aaataaaaaa aattagtcag
     3781 ccatgagctt ggcccattgc atacgttgta tccatatcat aatatgtaca tttatattgg
     3841 ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc
     3901 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     3961 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     4021 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     4081 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     4141 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     4201 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     4261 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     4321 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     4381 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     4441 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     4501 cctccataga agacaccggg accgatccag cctcccctcg aagcttacat gtggtaccga
     4561 gctcggatcc tgagaacttc agggtgagtc tatgggaccc ttgatgtttt ctttcccctt
     4621 cttttctatg gttaagttca tgtcatagga aggggagaag taacagggta cacatattga
     4681 ccaaatcagg gtaattttgc atttgtaatt ttaaaaaatg ctttcttctt ttaatatact
     4741 tttttgttta tcttatttct aatactttcc ctaatctctt tctttcaggg caataatgat
     4801 acaatgtatc atgcctcttt gcaccattct aaagaataac agtgataatt tctgggttaa
     4861 ggcaatagca atatttctgc atataaatat ttctgcatat aaattgtaac tgatgtaaga
     4921 ggtttcatat tgctaatagc agctacaatc cagctaccat tctgctttta ttttatggtt
     4981 gggataaggc tggattattc tgagtccaag ctaggccctt ttgctaatca tgttcatacc
     5041 tcttatcttc ctcccacagc tcctgggcaa cgtgctggtc tgtgtgctgg cccatcactt
     5101 tggcaaagca cgtgagatct gaattctgac actatgaagt gccttttgta cttagccttt
     5161 ttattcattg gggtgaattg caagttcacc atagtttttc cacacaacca aaaaggaaac
     5221 tggaaaaatg ttccttctaa ttaccattat tgcccgtcaa gctcagattt aaattggcat
     5281 aatgacttaa taggcacagc cttacaagtc aaaatgccca agagtcacaa ggctattcaa
     5341 gcagacggtt ggatgtgtca tgcttccaaa tgggtcacta cttgtgattt ccgctggtat
     5401 ggaccgaagt atataacaca ttccatccga tccttcactc catctgtaga acaatgcaag
     5461 gaaagcattg aacaaacgaa acaaggaact tggctgaatc caggcttccc tcctcaaagt
     5521 tgtggatatg caactgtgac ggatgccgaa gcagtgattg tccaggtgac tcctcaccat
     5581 gtgctggttg atgaatacac aggagaatgg gttgattcac agttcatcaa cggaaaatgc
     5641 agcaattaca tatgccccac tgtccataac tctacaacct ggcattctga ctataaggtc
     5701 aaagggctat gtgattctaa cctcatttcc atggacatca ccttcttctc agaggacgga
     5761 gagctatcat ccctgggaaa ggagggcaca gggttcagaa gtaactactt tgcttatgaa
     5821 ac