pGL4.33[Luc2p/SRE/Hygro]哺乳报告质粒

基本信息

原核抗性:	Amp
筛选标记:	Hyg
克隆菌株:	DH5α
培养条件:	LB/37℃

质粒简介

            pGL4.33[Luc2p-SRE-Hygro]哺乳报告质粒

质粒图谱

质粒序列

LOCUS       Exported                6112 bp ds-DNA     circular SYN 01-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.33[luc2P-SRE-Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, July 6, 2018 
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        179..210
                     /note="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             243..1892
                     /codon_start=1
                     /product="firefly luciferase"
                     /note="luciferase"
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             1896..2015
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /note="hPEST"
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    2067..2188
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        2382..2739
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      2590..2725
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             2770..3807
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     polyA_signal    3831..3879
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4206..4794)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4994..5854)
                     /codon_start=1
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5959..6007
                     /note="synthetic polyadenylation signal"
     misc_feature    6021..6112
                     /note="pause site"
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcaggatgtc catattagga catctaggat
       61 gtccatatta ggacatctag gatgtccata ttaggacatc taggatgtcc atattaggac
      121 atctaggatg tccatattag gacatctaga tctggcctcg gcggccaagc ttagacacta
      181 gagggtatat aatggaagct cgacttccag cttggcaatc cggtactgtt ggtaaagcca
      241 ccatggaaga tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg
      301 ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca
      361 tcgcctttac cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga
      421 gcgttcggct ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg
      481 tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg
      541 tggctgtggc cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca
      601 tcagccagcc caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc
      661 aaaagaagct accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg
      721 gcttccaaag catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg
      781 acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg
      841 gcagtaccgg attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca
      901 gtcatgcccg cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg
      961 tggtgccatt tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct
     1021 ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact
     1081 ataagattca atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc
     1141 tcatcgacaa gtacgaccta agcaacttgc acgagatcgc cagcggcggg gcgccgctca
     1201 gcaaggaggt aggtgaggcc gtggccaaac gcttccacct accaggcatc cgccagggct
     1261 acggcctgac agaaacaacc agcgccattc tgatcacccc cgaaggggac gacaagcctg
     1321 gcgcagtagg caaggtggtg cccttcttcg aggctaaggt ggtggacttg gacaccggta
     1381 agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg
     1441 gctacgttaa caaccccgag gctacaaacg ctctcatcga caaggacggc tggctgcaca
     1501 gcggcgacat cgcctactgg gacgaggacg agcacttctt catcgtggac cggctgaaga
     1561 gcctgatcaa atacaagggc taccaggtag ccccagccga actggagagc atcctgctgc
     1621 aacaccccaa catcttcgac gccggggtcg ccggcctgcc cgacgacgat gccggcgagc
     1681 tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg
     1741 actatgtggc cagccaggtt acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg
     1801 acgaggtgcc taaaggactg accggcaagt tggacgcccg caagatccgc gagattctca
     1861 ttaaggccaa gaagggcggc aagatcgccg tgaattctca cggcttccct cccgaggtgg
     1921 aggagcaggc cgccggcacc ctgcccatga gctgcgccca ggagagcggc atggatagac
     1981 accctgctgc ttgcgccagc gccaggatca acgtctaagg ccgcgactct agagtcgggg
     2041 cggccggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     2101 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2161 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     2221 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     2281 aatcgataag gatccgtttg cgtattgggc gctcttccgc tgatctgcgc agcaccatgg
     2341 cctgaaataa cctctgaaag aggaacttgg ttagctacct tctgaggcgg aaagaaccag
     2401 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     2461 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     2521 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     2581 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     2641 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     2701 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcgattc ttctgacact
     2761 agcgccacca tgaagaagcc cgaactcacc gctaccagcg ttgaaaaatt tctcatcgag
     2821 aagttcgaca gtgtgagcga cctgatgcag ttgtcggagg gcgaagagag ccgagccttc
     2881 agcttcgatg tcggcggacg cggctatgta ctgcgggtga atagctgcgc tgatggcttc
     2941 tacaaagacc gctacgtgta ccgccacttc gccagcgctg cactacccat ccccgaagtg
     3001 ttggacatcg gcgagttcag cgagagcctg acatactgca tcagtagacg cgcccaaggc
     3061 gttactctcc aagacctccc cgaaacagag ctgcctgctg tgttacagcc tgtcgccgaa
     3121 gctatggatg ctattgccgc cgccgacctc agtcaaacca gcggcttcgg cccattcggg
     3181 ccccaaggca tcggccagta cacaacctgg cgggatttca tttgcgccat tgctgatccc
     3241 catgtctacc actggcagac cgtgatggac gacaccgtgt ccgccagcgt agctcaagcc
     3301 ctggacgaac tgatgctgtg ggccgaagac tgtcccgagg tgcgccacct cgtccatgcc
     3361 gacttcggca gcaacaacgt cctgaccgac aacggccgca tcaccgccgt aatcgactgg
     3421 tccgaagcta tgttcgggga cagtcagtac gaggtggcca acatcttctt ctggcggccc
     3481 tggctggctt gcatggagca gcagactcgc tacttcgagc gccggcatcc cgagctggcc
     3541 ggcagccctc gtctgcgagc ctacatgctg cgcatcggcc tggatcagct ctaccagagc
     3601 ctcgtggacg gcaacttcga cgatgctgcc tgggctcaag gccgctgcga tgccatcgtc
     3661 cgcagcgggg ccggcaccgt cggtcgcaca caaatcgctc gccggagcgc agccgtatgg
     3721 accgacggct gcgtcgaggt gctggccgac agcggcaacc gccggcccag tacacgaccg
     3781 cgcgctaagg aggtaggtcg agtttaaact ctagaaccgg tcatggccgc aataaaatat
     3841 ctttattttc attacatctg tgtgttggtt ttttgtgtgt tcgaactaga tgctgtcgac
     3901 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     3961 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     4021 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4081 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4141 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4201 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4261 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4321 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4381 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4441 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4501 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4561 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4621 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4681 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4741 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4801 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4861 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4921 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagcggccg caaatgctaa
     4981 accactgcag tggttaccag tgcttgatca gtgaggcacc gatctcagcg atctgcctat
     5041 ttcgttcgtc catagtggcc tgactccccg tcgtgtagat cactacgatt cgtgagggct
     5101 taccatcagg ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt
     5161 tgtcagcaat gaaccagcca gcagggaggg ccgagcgaag aagtggtcct gctactttgt
     5221 ccgcctccat ccagtctatg agctgctgtc gtgatgctag agtaagaagt tcgccagtga
     5281 gtagtttccg aagagttgtg gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg
     5341 gtatggcttc gttcaactct ggttcccagc ggtcaagccg ggtcacatga tcacccatat
     5401 tatgaagaaa tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt aagttggccg
     5461 cggtgttgtc gctcatggta atggcagcac tacacaattc tcttaccgtc atgccatccg
     5521 taagatgctt ttccgtgacc ggcgagtact caaccaagtc gttttgtgag tagtgtatac
     5581 ggcgaccaag ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca catagcagta
     5641 ctttgaaagt gctcatcatc gggaatcgtt cttcggggcg gaaagactca aggatcttgc
     5701 cgctattgag atccagttcg atatagccca ctcttgcacc cagttgatct tcagcatctt
     5761 ttactttcac cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg
     5821 gaatgagtgc gacacgaaaa tgttggatgc tcatactcgt cctttttcaa tattattgaa
     5881 gcatttatca gggttactag tacgtctctc aaggataagt aagtaatatt aaggtacggg
     5941 aggtattgga caggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     6001 ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa
     6061 ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ct