pUC18-TMV病毒基因质粒

基本信息

复制子:	ori
质粒分类:	基因文库质粒；其它基因质粒；其它种源质粒
质粒大小:	9064bp
原核抗性:	氨苄青霉素Amp（100μg/ml）
克隆菌株:	Stbl3等大肠杆菌
培养条件:	37℃，有氧，LB

质粒简介

            pUC18-TMV质粒上有RdRp、MP、壳蛋白CP基因。

质粒图谱

质粒序列

LOCUS       Exported                9064 bp ds-DNA     circular SYN 15-AUG-2017
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..9064
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        404..421
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    432..487
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     misc_feature    489..6129
                     /label=RdRp+MP
     CDS             6132..6611
                     /codon_start=1
                     /label=CP
                     /note="壳蛋白CP基因"
                     /translation="MSYSITTPSQFVFLSSAWADPIELINLCTNALGNQFQTQQARTVV
                     QRQFSEVWKPSPQVTVRFPDSDFKVYRYNAVLDPLVTALLGAFDTRNRIIEVENQANPT
                     TAETLDATRRVDDATVAIRSAINNLIVELIRGTGSYNRSSFESSSGLVWTSGPAT"
     primer_bind     complement(6843..6859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6867..6883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6891..6921)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6936..6957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7245..7833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8004..8864)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8865..8969)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttaatacg actcactata
      421 gtatttttac aacaattacc aacaacaaca aacaacaaac aacattacaa ttactattta
      481 caattacaat ggcatacaca cagacagcta ccacatcagc tttgctggac actgtccgag
      541 gaaacaactc cttggtcaat gatctagcaa agcgtcgtct ttacgacaca gcggttgaag
      601 agtttaacgc tcgtgaccgc aggcccaagg tgaacttttc aaaagtaata agcgaggagc
      661 agacgcttat tgctacccgg gcgtatccag aattccaaat tacattttat aacacgcaaa
      721 atgccgtgca ttcgcttgca ggtggattgc gatctttaga actggaatat ctgatgatgc
      781 aaattcccta cggatcattg acttatgaca taggcgggaa ttttgcatcg catctgttca
      841 agggacgagc atatgtacac tgctgcatgc ccaacctgga cgttcgagac atcatgcggc
      901 acgaaggcca gaaagacagt attgaactat acctttctag gctagagaga ggggggaaaa
      961 cagtccccaa cttccaaaag gaagcatttg acagatacgc agaaattcct gaagacgctg
     1021 tctgtcacaa tactttccag acaatgcgac atcagccgat gcagcaatca ggcagagtgt
     1081 atgccattgc gctacacagc atatatgaca taccagccga tgagttcggg gcggcactct
     1141 tgaggaaaaa tgtccatacg tgctatgccg ctttccactt ctctgagaac ctgcttcttg
     1201 aagattcata cgtcaatttg gacgaaatca acgcgtgttt ttcgcgcgat ggagacaagt
     1261 tgaccttttc ttttgcatca gagagtactc ttaattattg tcatagttat tctaatattc
     1321 ttaagtatgt gtgcaaaact tacttcccgg cctctaatag agaggtttac atgaaggagt
     1381 ttttagtcac cagagttaat acctggtttt gtaagttttc tagaatagat acttttcttt
     1441 tgtacaaagg tgtggcccat aaaagtgtag atagtgagca gttttatact gcaatggaag
     1501 acgcatggca ttacaaaaag actcttgcaa tgtgcaacag cgagagaatc ctccttgagg
     1561 attcatcatc agtcaattac tggtttccca aaatgaggga tatggtcatc gtaccattat
     1621 tcgacatttc tttggagact agtaagagga cgcgcaagga agtcttagtg tccaaggatt
     1681 tcgtgtttac agtgcttaac cacattcgaa cataccaggc gaaagctctt acatacgcaa
     1741 atgttttgtc ctttgtcgaa tcgattcgat cgagggtaat cattaacggt gtgacagcga
     1801 ggtccgaatg ggatgtggac aaatctttgt tacaatcctt gtccatgacg ttttacctgc
     1861 atactaagct tgccgttcta aaggatgact tactgattag caagtttagt ctcggttcga
     1921 aaacggtgtg ccagcatgtg tgggatgaga tttcgctggc gtttgggaac gcatttccct
     1981 ccgtgaaaga gaggctcttg aacaggaaac ttatcagagt ggcaggcgac gcattagaga
     2041 tcagggtgcc tgatctatat gtgaccttcc acgacagatt agtgactgag tacaaggcct
     2101 ctgtggacat gcctgcgctt gacattagga agaagatgga agaaacggaa gtgatgtaca
     2161 atgcactttc agagttatcg gtgttaaggg agtctgacaa attcgatgtt gatgtttttt
     2221 cccagatgtg ccaatctttg gaagttgacc caatgacggc agcgaaggtt atagtcgcgg
     2281 tcatgagcaa tgagagcggt ctgactctca catttgaacg acctactgag gcgaatgttg
     2341 cgctagcttt acaggatcaa gagaaggctt cagaaggtgc tttggtagtt acctcaagag
     2401 aagttgaaga accgtccatg aagggttcga tggccagagg agagttacaa ttagctggtc
     2461 ttgctggaga tcatccggag tcgtcctatt ctaagaacga ggagatagag tctttagagc
     2521 agtttcatat ggcaacggca gattcgttaa ttcgtaagca gatgagctcg attgtgtaca
     2581 cgggtccgat taaagttcag caaatgaaaa actttatcga tagcctggta gcatcactat
     2641 ctgctgcggt gtcgaatctc gtcaagatcc tcaaagatac agctgctatt gaccttgaaa
     2701 cccgtcaaaa gtttggagtc ttggatgttg catctaggaa gtggttaatc aaaccaacgg
     2761 ccaagagtca tgcatggggt gttgttgaaa cccacgcgag gaagtatcat gtggcgcttt
     2821 tggaatatga tgagcagggt gtggtgacat gcgatgattg gagaagagta gctgtcagct
     2881 ctgagtctgt tgtttattcc gacatggcga aactcagaac tctgcgcaga ctgcttcgaa
     2941 acggagaacc gcatgtcagt agcgcaaagg ttgttcttgt ggacggagtt ccgggctgtg
     3001 ggaaaaccaa agaaattctt tccagggtta attttgatga agatctaatt ttagtacctg
     3061 ggaagcaagc cgcggaaatg atcagaagac gtgcgaattc ctcagggatt attgtggcca
     3121 cgaaggacaa cgttaaaacc gttgattctt tcatgatgaa ttttgggaaa agcacacgct
     3181 gtcagttcaa gaggttattc attgatgaag ggttgatgtt gcatactggt tgtgttaatt
     3241 ttcttgtggc gatgtcattg tgcgaaattg catatgttta cggagacaca cagcagattc
     3301 catacatcaa tagagtttca ggattcccgt accccgccca ttttgccaaa ttggaagttg
     3361 acgaggtgga gacacgcaga actactctcc gttgtccagc cgatgtcaca cattatctga
     3421 acaggagata tgagggcttt gtcatgagca cttcttcggt taaaaagtct gtttcgcagg
     3481 agatggtcgg cggagccgcc gtgatcaatc cgatctcaaa acccttgcat ggcaagatcc
     3541 tgacttttac ccaatcggat aaagaagctc tgctttcaag agggtattca gatgttcaca
     3601 ctgtgcatga agtgcaaggc gagacatact ctgatgtttc actagttagg ttaaccccta
     3661 caccagtctc catcattgca ggagacagcc cacatgtttt ggtcgcattg tcaaggcaca
     3721 cctgttcgct caagtactac actgttgtta tggatccttt agttagtatc attagagatc
     3781 tagagaaact tagctcgtac ttgttagata tgtataaggt cgatgcagga acacaatagc
     3841 aattacagat tgactcggtg ttcaaaggtt ccaatctttt tgttgcagcg ccaaagactg
     3901 gtgatatttc tgatatgcag ttttactatg ataagtgtct cccaggcaac agcaccatga
     3961 tgaataattt tgatgctgtt accatgaggt tgactgacat ttcattgaat gtcaaagatt
     4021 gcatattgga tatgtctaag tctgttgctg cgcctaagga tcaaatcaaa ccactaatac
     4081 ctatggtacg aacggcggca gaaatgccac gccagactgg actattggaa aatttagtgg
     4141 cgatgattaa aaggaacttt aacgcacccg agttgtctgg catcattgat attgaaaata
     4201 ctgcatcttt agttgtagat aagttttttg atagttattt gcttaaagaa aaaagaaaac
     4261 caaataaaaa tgtttctttg ttcagtagag agtctctcaa tagatggtta gaaaagcagg
     4321 aacaggtaac aataggccag ctcgcagatt ttgattttgt agatttgcca gcagttgatc
     4381 agtacagaca catgattaaa gcacaaccca agcaaaaatt ggacacttca atccaaacgg
     4441 agtacccggc tttgcagacg attgtgtacc attcaaaaaa gatcaatgca atatttggcc
     4501 cgttgtttag tgagcttact aggcaattac tggacagtgt tgattcgagc agatttttgt
     4561 ttttcacaag aaagacacca gcgcagattg aggatttctt cggagatctc gacagtcatg
     4621 tgccgatgga tgtcttggag ctggatatat caaaatacga caaatctcag aatgaattcc
     4681 actgtgcagt agaatacgag atctggcgaa gattgggttt tgaagacttc ttgggagaag
     4741 tttggaaaca agggcataga aagaccaccc tcaaggatta taccgcaggt ataaaaactt
     4801 gcatctggta tcaaagaaag agcggggacg tcacgacgtt cattggaaac actgtgatca
     4861 ttgctgcatg tttggcctcg atgcttccga tggagaaaat aatcaaagga gccttttgcg
     4921 gtgacgatag tctgctgtac tttccaaagg gttgtgagtt tccggatgtg caacactccg
     4981 cgaatcttat gtggaatttt gaagcaaaac tgtttaaaaa acagtatgga tacttttgcg
     5041 gaagatatgt aatacatcac gacagaggat gcattgtgta ttacgatccc ctaaagttga
     5101 tctcgaaact tggtgctaaa cacatcaagg attgggaaca cttggaggag ttcagaaggt
     5161 ctctttgtga tgttgctgtt tcgttgaaca attgtgcgta ttacacacag ttggacgacg
     5221 ctgtatggga ggttcataag accgcccctc caggttcgtt tgtttataaa agtctggtga
     5281 agtatttgtc tgataaagtt ctttttagaa gtttgtttat agatggctct agttgttaaa
     5341 ggaaaagtga atatcaatga gtttatcgac ctgacaaaaa tggagaagat cttaccgtcg
     5401 atgtttaccc ctgtaaagag tgttatgtgt tccaaagttg ataaaataat ggttcatgag
     5461 aatgagtcat tgtcagaggt gaaccttctt aaaggagtta agcttattga tagtggatac
     5521 gtctgtttag ccggtttggt cgtcacgggc gagtggaact tgcctgacaa ttgcagagga
     5581 ggtgtgagcg tgtgtctggt ggacaaaagg atggaaagag ccgacgaggc cactctcgga
     5641 tcttactaca cagcagctgc aaagaaaaga tttcagttca aggtcgttcc caattatgct
     5701 ataaccaccc aggacgcgat gaaaaacgtc tggcaagttt tagttaatat tagaaatgtg
     5761 aagatgtcag cgggtttctg tccgctttct ctggagtttg tgtcggtgtg tattgtttat
     5821 agaaataata taaaattagg tttgagagag aagattacaa acgtgagaga cggagggccc
     5881 atggaactta cagaagaagt cgttgatgag ttcatggaag atgtccctat gtcgatcagg
     5941 cttgcaaagt ttcgatctcg aaccggaaaa aagagtgatg tccgcaaagg gaaaaatagt
     6001 agtaatgatc ggtcagtgcc gaacaagaac tatagaaatg ttaaggattt tggaggaatg
     6061 agttttaaaa agaataattt aatcgatgat gattcggagg ctactgtcgc cgaatcggat
     6121 tcgttttaaa tatgtcttac agtatcacta ctccatctca gttcgtgttc ttgtcatcag
     6181 cgtgggccga cccaatagag ttaattaatt tatgtactaa tgccttagga aatcagtttc
     6241 aaacacaaca agctcgaact gtcgttcaaa gacaattcag tgaggtgtgg aaaccttcac
     6301 cacaagtaac tgttaggttc cctgacagtg actttaaggt gtacaggtac aatgcggtat
     6361 tagacccgct agtcacagca ctgttaggtg cattcgacac tagaaataga ataatagaag
     6421 ttgaaaatca ggcgaacccc acgactgccg aaacgttaga tgctactcgt agagtagacg
     6481 acgcaacggt ggccataagg agcgcgataa ataatttaat agtagaattg atcagaggaa
     6541 ccggatctta taatcggagc tctttcgaga gctcttctgg tttggtttgg acctctggtc
     6601 ctgcaacttg aggtagtcaa gatgcataat aaataacgga ttgtgtccgt aatcacacgt
     6661 ggtgcgtacg ataacgcata gtgtttttcc ctccacttaa atcgaagggt tgtgtcttgg
     6721 atcgcgcggg tcaaatgtat atggttcata tacatccgca ggcacgtaat aaagcgaggg
     6781 gttcgaatcc ccccgttacc cccggtaggg gcccaggtac cgagctcgaa ttcgtaatca
     6841 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6901 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6961 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7021 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7081 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7141 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7201 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7261 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7321 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7381 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7441 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7501 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7561 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7621 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7681 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7741 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7801 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7861 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7921 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7981 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8041 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8101 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8161 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8221 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8281 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8341 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8401 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8461 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8521 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8581 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8641 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8701 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8761 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8821 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8881 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8941 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     9001 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     9061 cgtc