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1 ml
predicted_size:null
Uniprot ID:P4626
description:Her2 (ERBB2) rabbit monoclonal antibody, clone SP3, Supernatant
clonality:Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...):Primary Antibodies
background:c-erbB-2 protein is a receptor tyrosine kinase of the c-erbB family. It is closely related in structure to the epidermal growth factor receptor. c-erbB-2 oncoprotein is detectable in a proportion of breast and other adenocarcinomas, as well as transitional cell carcinomas. In the case of breast cancer, expression determined by immunohistochemistry has been shown to be associated with poor prognosis.
immunogen:Recombinant protein encoding extracellular domain of human c-erbB-2
recommended_dilution:Immunohistochemistry on formalin-fixed paraffin embedded sections: dilute 1:100 in an ABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Breast carcinoma
predicted_size:null
buffer:State: Supernatant; State: Liquid tissue culture supernatant containing sodium azide as preservative
host_species:Rabbit
applications:IHC
reactivities:Human
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文献和实验Monoclonal Antibody Production
. (7) Centrifuge samples at 10,000g 10 min. (8) Pipet off the serum supernatant.Store at -20 ℃.This is your pre-bleed control. Hybridoma Fusion 2. For one fusion you will need (1) 8500 ml bottles of DMEM media (2) 3500 ml bottles of fetal calf serum (FCS
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridoma fusion: Immortalization of sensitized B lymphocytes from immune mice. Overview
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