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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
见产品外包装
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需确认
- CAS号:
480063-57-8
- 英文名:
p21CIP1, GST tagged human
- 保存条件:
−70°C
- 供应商:
嵘崴达
- 规格:
50 μg
p21CIP1, GST tagged human
recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
别名: CAP20, CDKN1, CDKN1A, MDA-6, P21, SDI1, WAF1
General description
CIP 1 (Cyclin-Dependent Kinase Inhibitor 1A) regulates cell cycle progression, terminal differentiation, and apoptosis. CIP1 was shown to be induced by p53 and to be a potent inhibitor of cyclin-dependent kinase (CDK) activity. DNA damage leads to increased expression of CIP1 in cyclin E-containing complexes and to an associated decrease in cyclin-dependent kinase activity. CIP1 is a critical downstream effector in the p53-specific pathway of growth control in mammalian cells.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
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文献和实验receptors or their domains in insect cells (6 ), Escherichia coli (7 ), or mammalian cells (8 ), has facilitated their purification. Purification with histidine (His)-tagged proteins is advantageous, because, unlike protein tags, such as glutathione S
Gene Targeting in Cultured Human Cells
control genes have been studied using human somatic-cell gene targeting, including p21WAF1/CIP1 , p53, 14-3-3σ, and (ataxia-telangiectasia and Rad3 [ATR] ) (2 –5 ).
Identifying the subcellular compartment of a protein is an important step toward assigning protein function. Starting with a clone containing the open reading frame (ORF) of interest, it is possible to attach a variety of short amino acid
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