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| 对精确模型的慢性、急性或间歇性缺氧或高氧状态, 提供了氧分布控制。 The only complete system available for hypoxic/hyperoxic tissue culture and live animal studies that allow CO2 incubation AND sample manipulation conveniently in a heated oxygen controlled atmosphere.
Through a strategically positioned oxygen sensor and controller the Coy Hypoxic Glove Box can maintain a uniform gas concentration throughout the chamber and optimum control. As gases are required in the glove box, they are introduced through a unique gas tube shower system which, along with the internal fan, provides the circulation required for uniform gas distribution and fast response of the controller. To allow for the relief of pressure induced by gas addition, special check/bleed valves are installed in the glove box.
Temperature Control (option) Units can either be heated or unheated or unheated with a separate Incubator. A solid state heater provides reliable, stable temperature control with digital display and controls in both our Incubators and Heated Glove Boxes.
CO 2 Control (option) All COY Hypoxic units can be equipped with optional CO2 Controls. A revolutionary controller and sensor, allows for precise control and monitoring of CO2 gases inside the glove box. The sensor relays information to the controller which then opens the appropriate Solenoid controlling a CO2 or N2 Gas to equilibrate the CO2 levels inside the glove box. The digital display allows the user to monitor the CO2 Levels constantly.
Humidity Control (option) COY offers a few different ways to control the humidity of the glove box depending on the application. Some heated units have a built in solid state dehumidifier with digital displays and controls. Typically the Solid State Automatic Dehumidifier is recommended but for some low volume/small size animals a more affordable desiccant drying system may be utilized. Cell Culture units typically are provided with an optional Humidified Incubation Box (high humidity box within the glove box) to prevent samples from drying out however since these are not air tight the moisture eventually escapes into the glove box atmosphere so the Solid State Dehumidifier or Desiccant drying system is used to maintain ideal relative humidity.
Cell Culture Units Cultures are incubated in the heated, humidified, gas controlled (up to 100% O2 ) incubation box which resides inside the glove box. The glove box is heated and gas regulated, but not humidified. This allows samples to be analyzed with delicate equipment that would be damaged by high humidity. More importantly, controlled oxygen conditions are maintained in the sample analysis area. Tests that could not be performed with a bench top enclosure and CO2 incubator because of the biologically fast reaction to ambient oxygen can now be performed. Live Animal Containment Depending on the labs animal care requirements the units can be heated or unheated. Most units for live animal containment will also require the COY Animal Filtration System to remove the animal waste byproducts, these units can be automatic or manual, with the manual based on a simple protocol the lab establishes, automatic units are based off the CO2 levels. The Animal Filtration system is a closed loop circuit with a pump drawing the glove box atmosphere out and through the filters (Carbolime and activated carbon) and back into the glove box. Airlock Sizes for Live Animal Containment Due to the variety of number and size of animals kept in these glove boxes COY manufactures each unit to specifically match a labs cage size to the airlock providing optimum efficiency.
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文献和实验结果表明光基因可以在体表达于动物的甲状旁腺,进行光调控可以有效抑制甲旁亢动物模型的 PTH 分泌。 研究人员还研发了一套钙响应自动光调控系统:利用钙电极来感受胞外钙浓度变化,当其浓度高于生理浓度时,钙电极将信号传入控制器打开光刺激器,抑制表达光基因的主细胞的 PTH 分泌。这套自动化系统能够帮助甲状旁腺细胞自动响应胞外钙浓度变化,进而实现对 PTH 的生理性调控。 更重要的是通过节律性光抑制 PTH 分泌可有效调节骨重塑过程,促进骨生成并抑制骨吸收。光调控甲旁腺组织后,小鼠松质骨的成骨细胞数量增加
Nature 子刊:高彩霞团队开发可预测的精细下调目标基因蛋白表达的新方法
降。OsDLT 基因编码 GRAS 蛋白家族成员,参与了水稻油菜素内酯信号转导途径,调控水稻株高、分蘖数、种子大小等多个重要农艺性状。该研究中以 OsDLT 基因为靶标,通过编辑 OsDLT 基因的 5'UTR,获得了一组具有不同叶夹角、株高和分蘖数的突变体,且突变体的表型变化趋势与瞬时系统预测结果一致。 通过编辑 uORF 培育具有目标性状的水稻植株。(a, b) 从头创制新的 uORFs (a) 或延伸内源 uORFs (b) 抑制目标基因的蛋白表达。(c) 通过产生一系列具有不同抑制
gliomagenesis via neuronal IGF1 的原创性研究成果,对上述问题进行了解答,首次明确 嗅觉感知可以通过激活对应功能神经环路的活动直接调控恶性胶质瘤发生,揭示了外部环境刺激可能是一种新的胶质瘤诱发因素,同时为胶质瘤的临床诊治提供新的思路和靶点。 研究团队首先构建并系统分析了一个模拟成人少突胶质前体细胞(OPC)作为细胞起源的原发胶质瘤小鼠遗传学模型(简称 CKO 模型),发现在该模型中胶质瘤最初发生的位置具有一定的位置偏好性:肿瘤总是先出现在大脑嗅球的一个亚解剖结构-突触小球层(GL
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