Matrices listed below were spiked with certain level of GLB1 and the recovery rates were calculated by comparing the measured value to the expected amount of GLB1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-98
93
EDTA plasma(n=5)
86-103
95
heparin plasma(n=5)
92-100
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GLB1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.