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文献和实验Dissociation of Cells from Primary Tissue
solution without calcium or magnesium (1 ml of trypsin for every 100 mg of tissue). 3.Incubate at 4°C for 6 to 18 h to maximize penetration of the enzyme with little trypsin activity. 4.Decant and discard the trypsin
. Incubate for 15 min at 37 °C. Rinse each flask x 2 with 10 ml DPBS (without Ca or Mg). 6) Add 4 ml of Trypsin to each flask. Incubate at 37 °C for 3 min. Neutralize with 8 ml of Trypsin Neutralization Solution. 7) Wash
Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
Approximately 100 μl of radiolabeled DNA 15 μl of 10X Restriction Enzyme Buffer 15 μl of 10 mM DTT 1.5 μl of 20 mg/ml BSA 20 Units of Restriction Enzyme ddH2 O to give an approximate final volume of 150 μl
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