Matrices listed below were spiked with certain level of GDF5 and the recovery rates were calculated by comparing the measured value to the expected amount of GDF5 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
96
EDTA plasma(n=5)
89-94
91
heparin plasma(n=5)
94-104
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GDF5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.