Matrices listed below were spiked with certain level of NT-3 and the recovery rates were calculated by comparing the measured value to the expected amount of NT-3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-98
91
EDTA plasma(n=5)
93-105
100
heparin plasma(n=5)
86-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.