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- merck millipore
- BSS-011-D
- 2025年07月08日
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文献和实验Procedures for In Vitro Production of Bovine Embryos
. The following media should be prepared on day -1 so that they are ready for the next day (Day 0) HEPES-TL and HEPES-TALP IVF-TL and IVF-TALP Sp-TL and Sp-TALP 90% Percoll2. Instructions for preparing media are available
consists of 320 mM sucrose and 5 mM HEPES, pH 7.4 (adjusted with NaOH). It can be stored at 4 °C and used within 1 week after preparation. Homogenization buffer The solution consists of 250 mM sucrose and 3 mM
M199 + 35g NaHCO3 + 25mM Hepes + 5 mM glutamine + 50 ug/ml Gentamycin.1M Hepes: 119.1 g Hepes + 500 ml dH2 O.Media A: 1X HBSS (10X:50ml) + 10 mM Hepes (1M: 5 ml) + 5 mM EDTA (0.5M: 5ml) + 2.5 % FBS (12.5 ml) in 500 ml.Media B: RPMI1640 470 ml + Gent
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