- ¥1148
- stemcell
- 75011.1
- 美国
- 2026年01月06日
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75011.1
概要
GloCell™ Fixable Viability Dye Violet 540 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.GloCell™ Fixable Viability Dye Violet 540 is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 540 nm that can be detected using the 525/50 band pass filter used to detect AmCyan.
Application:
流式细胞术; 免疫荧光
Brand:
GloCell
Area of Interest:
血管生成细胞研究; 抗体制备; 癌症研究; 细胞系制备; 嵌合体; 疾病模型; 药物发现和毒性测试; 内皮细胞生物学; 上皮细胞生物学; HIV; HLA; 杂交瘤生成; 免疫学; 传染病; 代谢; 有丝分裂; 神经科学; Organoids; 呼吸系统研究; 干细胞生物学; T细胞工程; 移植研究
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文献和实验LIVE/DEAD® Violet Viability/Vitality Kit
than its parent compound. The calcein violet AM ester is colorless and non fluorescent until hydrolyzed. The polyanionic dye calcein violet is well retained within live cells, producing an intense uniform violet fluorescence in live cells (ex/em 400/452 nm
Vybrant® DyeCycle Violet Stain
(SP) technique; the efflux can be blocked with verapamil, fumitermorgin C, or other such blocking agents, to prevent dye efflux for accurate DNA content analysis in these stem cells Vybrant ® DyeCycle™ Violet Staining Protocol
in multicolor immunophenotyping experiments that incorporate a LIVE/DEAD® fixable dye by following the protocol outlined below: 1) Gently vortex the ArC™ Amine Reactive Beads Kit and the AbC™ anti-Rat/Hamster Bead Kit components for 30 seconds
