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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
欣博盛
- 检测方法:
夹心法ELISA
- 适应物种:
human
- 样本:
血清,血浆等
- 规格:
1*96wells
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文献和实验a simple ELISA method to detect human anti-antibody response.
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
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