- $590
- LSBio
- 0.05 - 40 ng/ml
- LS-F531
- 美国
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
0.05 - 40 ng/ml
- 供应商：
康朗生物
- 规格：
96T
Human IgG1 ELISA Kit (Sandwich ELISA)
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Product Description
LS-F531 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human IgG1 in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of IgG1 as low as 0.05 nanograms per millilter.Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
IgG1
Reactivity
Human
Specificity
The antibody pair provided in this kit recognizes human IgG1.
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Sample Dilution
10000000 fold
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.05 - 40 ng/ml
Sensitivity
0.05 ng/ml
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Protocol
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl standard or sample to each well.
- Incubate 2.5 hours at room temperature or over night at 4°C.
- Add 100 µl prepared biotin antibody to each well.
- Incubate 1 hour at room temperature.
- Add 100 µl prepared Streptavidin solution.
- Incubate 45 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well.
- Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well.
- Read at 450 nm immediately.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Sandwich ELISA Platform Overview
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