Rat Insulin C-Peptide ELISA Ki

Rat Insulin C-Peptide ELISA Kit (Sandwich ELISA)

Catalog
Size
Price
LS-F32168-1
1 plate
$695 USD
Request Bulk
Add to Wish List
Add to Cart

Print This Page
Technical Help
Review This Product
SpecificationsPublicationsReviewsImages

Product Description

LS-F32168 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat Insulin C-Peptide in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of Insulin C-Peptide as low as 46.9 picograms per milliliter.

---

Specifications

Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
Insulin C-Peptide
Reactivity
 
Rat
Manual
 

Intended Sample Types
 
Plasma, Serum, Tissue Homogenates
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
78 - 5000 pg/ml
Sensitivity
 
46.9 pg/ml
Precision
 
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
 
Store at 4°C. Stable for 6 months.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Background
 
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- C-P antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-C-P antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the C-P amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of C-P can be calculated.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.

Popular Insulin C-Peptide Elisa Kits

Mouse Insulin C-Peptide ELISA Kit (Competitive EIA) - LS-F25003
Reactivity: Mouse
Range: 4.94-400 pg/ml

Rat Insulin C-Peptide ELISA Kit (Competitive EIA) - LS-F13005
Reactivity: Rat
Range: 78-5000 pg/ml

Dog Insulin C-Peptide ELISA Kit (Competitive EIA) - LS-F25002
Reactivity: Dog
Range: 98.77-8000 pg/ml

Human Insulin C-Peptide ELISA Kit (Sandwich ELISA) - LS-F10006
Reactivity: Human
Range: 0.7-10 ng/ml

Cynomolgus Insulin C-Peptide ELISA Kit (Sandwich ELISA) - LS-F10142
Reactivity: Cynomolgus
Range: 0.7-10 ng/ml
More Insulin C-Peptide Products

---

Publications (0)

Submit a Publication

---

Customer Reviews (0)

Submit a Review
Top of Page

---

Images

 

 

Sandwich ELISA Platform Overview