- $1315
- LSBio
- 0.234 - 60 µg/ml
- LS-F54121
- 美国
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Chicken
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
0.234 - 60 µg/ml
- 供应商：
康朗生物
- 规格：
96T
Chicken IgA, Secretory ELISA Kit (Competitive EIA)
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Product Description
LS-F54121 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Chicken IgA, Secretory in samples of Plasma and Serum. It is based upon a Competitive EIA assay principle and can be used to detect levels of IgA, Secretory as low as 0.058 micrograms per millilter.Specifications
TypeCompetitive EIA (enzyme immunoassay) kit
Target
IgA, Secretory
Reactivity
Chicken
Specificity
This assay has high sensitivity and excellent specificity for detection of chicken SIgA. No significant cross-reactivity or interference between chicken SIgA and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between chicken SIgA and all the analogues, therefore, cross reaction may still exist.
Manual
Intended Sample Types
Plasma, Serum
Sample Size
50 - 100 µl
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.234 - 60 µg/ml
Sensitivity
0.058 µg/ml
Assay Time
2.5 h
Precision
Intra-Assay: CV<8% / Inter-Assay: CV<10%
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Competition ELISA Platform Overview
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠IgA 定量 EIA 试剂盒使用说明 原理 本实验采用双抗体夹心 ELISA 法。用抗小鼠 IgA 单抗包被于酶标板上，标准品和样品中的 IgA 与单抗结合，加入酶标抗体，形成免疫复合物连接在板上，加入酶底物TMB ，出现蓝色，加终止液硫酸，颜色变黄，在 4 50 nm 处测 OD
。非均相 EIA 需先进行游离的和结合的标记物的分离。如前所述，固相载体可用作一种分离手段。这种固相酶免疫测定方法在 1971 年最初建立时称为酶联免疫吸附剂测定（enzymelinked immunosorbent assay），简称 ELISA，在国内有译作酶联免疫吸附试验的，虽然含义不完全确切，但已习用。
其对底物作用的活力，因而测出的酶活力直接反映游离的酶标记物。均相EIA在临床检验中较少应用。非均相EIA需先进行游离的和结合的标记物的分离。如前所述，固相载体可用作一种分离手段。这种固相酶免疫测定方法在1971年最初建立时称为酶联免疫吸附剂测定（enzyme linked immunosorbent assay），简称ELISA，在国内有译作酶联免疫吸附试验或酶标，已习用。
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