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文献和实验. During apoptosis, PS is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2 -dependent affinity for PS and therefore can be used as a probe for detecting apoptosis. 实验步骤 Example
Apoptosis Measurement by Annexin V Staining
assay. Recently, PARP cleavage (7 ) and annexin V (8 –11 ) have claimed more specificity. PARP cleavage occurs early in the response as a result of the activity of caspase-3 and correlates well with chromatin condensation (12 ). PARP cleavage
Purification of Annexin V and Its Use in the Detection of Apoptotic Cells
, but not necrosis, allowing differentiation between the two types of cell death. In this chapter we detail two methods for the purification of annexin V. The first is an untagged recombinant protein using a three step Fast Protein Liquid Chromatography (FPLC) method
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