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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Annexin V-AF 488 conjugate, 5 ug
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
5ug
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文献和实验染料,它不能透过完整的细胞膜,但在凋亡中晚期的细胞和死细胞,PI能够透过细胞膜而使细胞核红染。因此将Annexin-V与PI匹配使用,就可以将凋亡早晚期的细胞以及死细胞区分开来。方法1、悬浮细胞的染色:将正常培养和诱导凋亡的悬浮细胞(0.5~1×106)用PBS洗2次,加入100ul Binding Buffer和FITC标记的Annexin-V(20ug/ml)10ul,室温避光30min,再加入PI(50ug/ml)5ul,避光反应5min后,加入400ul Binding Buffer,立即
. During apoptosis, PS is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2 -dependent affinity for PS and therefore can be used as a probe for detecting apoptosis. 实验步骤 Example
Apoptosis Measurement by Annexin V Staining
assay. Recently, PARP cleavage (7 ) and annexin V (8 –11 ) have claimed more specificity. PARP cleavage occurs early in the response as a result of the activity of caspase-3 and correlates well with chromatin condensation (12 ). PARP cleavage
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