序列: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln Biological ActivityProSpec’s Leptin Antagonist Triple Mutant Mouse Recombinant is capable of inhibiting Leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human Leptin receptor. It also inhibits various Leptin effects in several in vitro bioassays.
溶解建议: 提供的小鼠瘦素三倍体拮抗剂,PEG修饰(mLeptin tA PEG)产品为白色冻干粉末;该冻干粉末是从含0.65mg/ml小鼠瘦素三倍体拮抗剂,PEG修饰(mLeptin tA PEG)的0.003mM NaHCO3溶液中冻干产生的.我们建议加无菌的水或无菌的0.4% NaHCO3(pH 8-9)以配制工作储存液到100ug/ml,后继实验过程中可根据实验要求进一步稀释.
保存建议: 小鼠瘦素三倍体拮抗剂,PEG修饰(mLeptin tA PEG)冻干粉末在室温可保存数周,或置于-18℃冻存用于长期保存.重新溶解后过滤灭菌浓度大于100ug/ml的溶液可以在4℃冷藏保存或室温下数周,低于此浓度的蛋白需要加载体蛋白如0.1% HSA或 BSA;如果想延长保存时间,建议分装后置于-18℃冻存.请避免反复冻融.
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