重组人GLI发病机理相关蛋白2

产品英文名称：
Recombinant Wheat Gliadin

英文简称:
Gliadin

中文名称:
重组麦醇溶蛋白

背景资料:
Wheat Gliadin and related gluten components from barley， rye and possibly oats can cause an abnormal immune response called Celiac disease which is a chronic gastrointestinal disorder. Celiac disease characteristics are flattening of the jejunal mucosa and intestinal lesions of variable severity in hereditarily inclined individuals. Even though Celiac disease is not a classic autoimmune disease it is related to anti-tissue transglutaminase antibodies and gliadin antibodies tests are most recommended in scrGliadin is a cDNA coding for an epitope-carrying fragment of a wheat gamma-gliadin isoform， having a molecular mass of 18，616 Dalton （high proline content and the low pI are likely causes for the observed discrepancy between calculated molecular weight and the observed electrophoretic mobility of approx. 47 kDa on standard SDS-PAGE）， pH 4.6. By sequence design the epitopes correspond to the deamidated neo-epitopes， which in the natural antigen are formed by transglutaminase-mediated glutamine side chain deamidation. Gliadin protein is fused to a hexa-histidine purification tag.ning populations at risk for CD and other gluten-sensitive enteropathies. In the past， serologic tests for gliadin antibodies usually were not very precise and were not enough for accurate diagnosis due to missing deamidated epitopes within the authentic gliadin fraction traditionally used in diagnostic test kits. ProSpec’s deamidated Gliadin isoform matches to the deamidated neo-epitopes， which in the natural antigen are formed by transglutaminase-mediated glutamine side chain deamidation.

产品描述:
Gliadin is a cDNA coding for an epitope-carrying fragment of a wheat gamma-gliadin isoform， having a molecular mass of 18，616 Dalton （high proline content and the low pI are likely causes for the observed discrepancy between calculated molecular weight and the observed electrophoretic mobility of approx. 47 kDa on standard SDS-PAGE）， pH 4.6. By sequence design the epitopes correspond to the deamidated neo-epitopes， which in the natural antigen are formed by transglutaminase-mediated glutamine side chain deamidation. Gliadin protein is fused to a hexa-histidine purification tag.
来源宿主:
大肠杆菌中重组表达

序列:


溶解建议:
提供的重组麦醇溶蛋白产品形式为经无菌过滤处理的澄清溶液.

保存建议：
重组麦醇溶蛋白产品在4℃保存请2 - 4周内将使用；-20℃可长期保存.产品若长期保存，建议添加载体蛋白（0.1% HSA或BSA）.避免反复冻融.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。