背景资料: LIN28 plays an important role as a ’translational enhancer’, leading specific mRNAs to polysomes and therefore increasing the competence of protein synthesis. LIN28 is a marker of undi大肠杆菌中重组表达erentiated human embryonic stem cells and it enhances the e大肠杆菌中重组表达iciency of the formation of induced pluripotent stem (iPS) cells from human fibroblasts. LIN28 binds to the let-7 pre-miRNA and blocks production of the mature let-7 microRNA in mouse embryonic stem cells. Overexpression of LIN28 is associated with human germ-cell tumors.
产品描述: Recombinant Human LIN28 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 191 amino acids (42-209) and having a molecular mass of 21.1 kDa.LIN28 is expressed with a 23 amino acid His tag fused at N-Terminus and purified by proprietary chromatographic techniques.
来源宿主: 大肠杆菌中重组表达
溶解建议: Sterile filtered colorless solution. The LIN28 protein solution contains 20mM Tris-HCl, pH-8, 10% glycerol and 0.1M NaCl.
保存建议: Store at 4℃ if entire vial will be used within 2-4 weeks.Store, frozen at -20℃ for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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