人低密度脂蛋白

产品英文名称：
Recombinant Mouse Lipopolysa重组小鼠人脂多糖结合蛋白arid Binding Protein

英文简称:
mLBP

中文名称:
重组小鼠人脂多糖结合蛋白

背景资料:
Lipopolysaccharides （LPS） are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein （aka LBP） is a plasma protein which facilitates the diCHO中国仓鼠卵巢细胞中重组表达usion of bacterial LPS （endotoxin）. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein （BPI）， LBP binds LPS and interacts with the CD14 receptor， most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally and functionally related proteins， including BPI， plasma cholesteryl ester transfer protein （CETP）， and phospholipid transfer protein （PLTP）. The LBP gene is found on chromosome 20， directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aregates to HDL particles， to phospholipid bilayers， and to a binding site on soluble CD14 （sCD14）. sCD14 is capable of spLBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before tranfection the complete mouse LBP cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal aCHO中国仓鼠卵巢细胞中重组表达inity purification with Talon and controlled by SDS page. Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split oCHO中国仓鼠卵巢细胞中重组表达. up to 2ug/ml LBP mediates binding of FITC-LPS （0.5ug/ml） to CD14+CHO transfectants （2 x 1，000，000/ml）.ding up the transfer by receiving an LPS monomer from an LPS aregate， and then yielding it to an HDL particle， therefore acting as a soluble "shuttle" for an insoluble lipid.

产品描述:
LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before tranfection the complete mouse LBP cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal aCHO中国仓鼠卵巢细胞中重组表达inity purification with Talon and controlled by SDS page. Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split oCHO中国仓鼠卵巢细胞中重组表达. up to 2ug/ml LBP mediates binding of FITC-LPS （0.5ug/ml） to CD14+CHO transfectants （2 x 1，000，000/ml）.
来源宿主:
CHO中国仓鼠卵巢细胞中重组表达

序列:


溶解建议:
Sterile Filtered White lyophilized （freeze-dried） powder. Recombinant Mouse LBP was lyophilized from a protein solution （1 mg/ml） containing phosphate-buffered saline， pH 7.2. It is recommended to reconstitute the lyophilized LBP in sterile 18MΩ-cm H2O not less than 100ug/ml， which can then be further diluted to other aqueous solutions.

保存建议：
Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon reconstitution LBP should be stored at 4℃ between 2-7 days and for future use below -18℃. Please prevent freeze-thaw cycles.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。