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文献和实验distilled water were mixed together. 4) Then additional tubes were set up comprising standard DNA (lambda or calf thymus DNA are routinely used in calibration. In general eukaryotic DNA is best used for eukaryotic samples.) c. 25 ng/µl 0.5µl calf thymus
96-well RNA In Situ Hybridization Protocol
is described in section IV. 1. Place 5ul of linearized plasmid (100 - 500ng) into 96 well plate. (The CK cDNA miniprep DNA was linearized with Apa I). If restriction buffer is compatible with the polymerase buffer, 5ul of restriction digest can be used
96-well RNA In Situ Hybridization Protocol
vendor information is described in section IV.1. Place 5μlof linearized plasmid (100 - 500ng) into 96 well plate. (The CK cDNA miniprep DNA was linearized with Apa I). If restriction buffer is compatible with the polymerase buffer, 5μlof restriction
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