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- 文献和实验
- 技术资料
- 保存条件:
2-8°C
- 保质期:
1年
- 英文名:
GroEL
- 库存:
999
- 供应商:
南京赛泓瑞
- 规格:
/5ug/20ug/1mg
Recombinant Human PM/Scl 100
英文简称:
PM/Scl 100
中文名称:
重组人PM/Scl100蛋白
背景资料:
PM/Scl 100 is a nucleolar protein and represents one crucial component of the so-called "PM/Scl complex" or "nuclear exosome", which is an RNA-processing complex consisting of a number of exoribonucleases (not to be confused with certain multivesicular bodies which are shed by cultured cells into the culture fluid and are also called exosomes). Autoantibodies against PM/Scl 100 occur in patients su昆虫Sf9细胞中重组表达ering from polymyositis, scleroderma or polymyositis/scleroderma overlap syndromes. While antibodies to other components of the exosome complex (PM/Scl 75, several Rrp proteins) can be found in some sera, practically all patients with autoantibodies against the PM/Scl complex are positive for PM/Scl 100 antibodies, making this specificity an important marker. The presence of PM/Scl 100 autoantibodies is a comparatively "good" prognostic sign in polymyositis/scleroderma overlap syndrome unlike the poor prognosis sPM/Scl 100 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 120 kDa. PM/Scl 100 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.n when other myositis and systemic sclerosis specific autoantibodies are present.
产品描述:
PM/Scl 100 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 120 kDa. PM/Scl 100 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
来源宿主:
昆虫Sf9细胞中重组表达
序列:
溶解建议:
Sterile Filtered clear solution. PM/Scl 100is supplied at a concentration of 0.8mg/ml in 20mM HEPES, pH-7.5, 100mM NaCl, 0.01mM EDTA, 0.02% SDS.
保存建议:
Store at 4℃ if entire vial will be used within 2-4 weeks. Store, frozen at -20℃ for longer periods of time. Avoid multiple freeze-thaw cycles.
其他简介:
ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。
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文献和实验摘 要 3-磷酸甘油醛脱氢酶折叠中间体与分子伴侣GroEL的“半位结合”模式 “分子伴侣”的发现和鉴定改变了蛋白质折叠研究的经典概念,开辟了崭新的蛋白质生物合成研究的时代,为解译第二遗传密码并最终全面阐明分子生物学中心法则奠定了基础。分子伴侣功能的研究是蛋白质折叠研究领域中最活跃的前沿,其中分子伴侣与其帮助的靶蛋白之间的识别、相互作用以及解离又是分子伴侣作用机制研究最根本的内容。李剑研究了当前最受注意的分子伴侣GroEL与同源四聚体的3-磷酸甘油醛脱氢酶(GAPDH)、单肽链的溶菌
of scaffolding effects and ATP-driven conformational changes that mediate the temporary unfolding and subsequent refolding of protein substrates. DnaK and GroEL are the two major Escherichia coli chaperones. They belong to the HSP70 and HSP60 families of proteins
出了与NusA标签融合后的活性。 NusA标签提高蛋白可溶性的可能机制 Houry(1999)等揭示NusA蛋白是分子伴侣GroEL在体内的必须底物。而GroEL与其共作用因子GroES是大肠杆菌唯一的在所有生长条件下必需的分子伴侣系统。Douette等(2005)研究了融合蛋白NusA-UCP1(uncoupling protein 1)的可溶产量。UCP1是一种线粒体膜蛋白。这些作者发现16℃培养时
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