bHolo Transferrin

产品英文名称：
Recombinant Human B-Cell Leukemia/Lymphoma 2

英文简称:
Bcl-2

中文名称:
重组人B细胞淋巴瘤因子2

背景资料:
BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2， such as in the case of translocation of BCL2 to Ig heavy chain locus， is thought to be the cause of follicular lymphoma. Two transcript variants， produced by alternate splicing， di大肠杆菌中重组表达er in their C-terminal ends.

产品描述:
Bcl-2 Human Recombinant produced in E.Coli is a single， non-glycosylated polypeptide chain containing amino acids 1-206. The wild type Bcl-2 is missing 12 amino acids from C-terminus. C-terminus is fused to His-Tag. C-terminus his-tag， mimics the deleted C-terminus membrane domain thus maintaining its biological activity. Bcl-2 is purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达

序列:


溶解建议:
Sterile Filtered White lyophilized （freeze-dried） powder. The protein contains 10mM Tris-HCL pH-8， 1mM EDTA and 250mM NaCl. Suspend Bcl-2 in 100ul of 0.5M Acetic acid， over night at 4°C. Dilute 10 fold into selected buffer system. BCL-2 has tendency to form intramolecular disulfide bond， 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel， 10mM DTT is recommended.

保存建议：
Lyophilized Bcl-2 although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon reconstitution Bcl-2 should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。