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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20
- 保质期:
1年
- 英文名:
Nick translation DNA labeling system
- 库存:
大量
- 供应商:
欣博盛
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1*50Reactions
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文献和实验DNA labeling by nick translation
2.5 µl EDTA (0.5 M, pH 8.0) and 2.5 µl SDS (20%) to stop the reaction, keep the probes at -20℃ until hybridization Optimal fragment length after nick translation DNA after agarose gel
Nick Translation and Random Hexamer Labeling of DNA
Labeled nucleotides (radioactive or fluorescent) can be incorporated efficiently into double-stranded DNA by nick translation. Nick translation works by using DNase and DNA polymerase I enzymes. DNase cuts one strand of the DNA, exposing 5′
Nick translation was the first method devised for the in vitro labeling of DNA (1 ). During the reaction the DNA to be labeled is nicked by DNase I yielding a free 3′ hydroxyl end. DNA polymerase I then adds a new nucleotide to this end
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