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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
参照说明书
- 英文名:
Heavy Meromyosin Protein (HMM Fragment)
- 库存:
1
- 供应商:
北京博蕾德生物科技有限公司
- 规格:
1x100ug/Largequantities
| 规格: | 1x100ug | 产品价格: | 询价 |
|---|---|---|---|
| 规格: | Largequantities | 产品价格: | 询价 |
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文献和实验The N-terminal and C-terminal portions of the heavy chain fragment C from botulinum neurotoxin serotype C [rBoNT(HC )] were expressed in Pichia pastoris and purified by ion-exchange chromotography (IEC). The N-terminal fragment, rBoNTC(Hc )-N
Detection and Visualization of Protein Interactions with Protein Fragment Complementation Assays
as biologically relevant and eliminating false-positive interactions immediately. It was with these challenges in mind that our laboratory developed the protein-fragment complementation assay (PCA) strategy. In this strategy, the gene for an enzyme is rationally
by a two- or three-step procedure. The method can insert any fragment into small or large episomes, without the need of an antibiotic selection gene. We have humanized the mouse luteinizing hormone receptor gene (Lhcgr ) by inserting a ~55 kb fragment from a BAC clone
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