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- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Human
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
15.625 - 1000 pg/ml
- 供应商:
康朗生物
- 规格:
96T
Human IL8 / Interleukin 8 ELISA Kit (CLIA)
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Product Description
LS-F29103 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human IL8 / Interleukin 8 in samples of Plasma and Serum. It is based upon a Sandwich CLIA assay principle and can be used to detect levels of IL8 / Interleukin 8 as low as 9.375 picograms per milliliter.Specifications
TypeCLIA (chemiluminescent immunoassay) kit
Target
IL8 / Interleukin 8
Reactivity
Human
Manual
Intended Sample Types
Plasma, Serum
Format
12 x 8-Well Microstrips
Detection
Chemiluminescent
Measurement
Quantitative
Detection Range
15.625 - 1000 pg/ml
Sensitivity
9.375 pg/ml
Precision
Intra-Assay: CV<15% Inter-Assay: CV<15%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About IL8 / Interleukin 8
P10145 NM_000584 NP_000575.1
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Sandwich CLIA Platform Overview
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文献和实验预期应用本试剂盒运用双抗体夹心 ELISA 法定量测定人组织匀浆、细胞裂解液和其他生物液体中 IL8Ra 含量。试剂盒内容试剂名称数量试剂名称数量96孔板(预包被)196孔板覆膜2标准品2标准品稀释液1×20mL检测溶液A1×120μL检测稀释液A1×12mL检测溶液B1×120μL检测稀释液B1×12mLTMB底物1×9mL终止液1×6mL浓洗涤液(30×)1×20mL使用说明书1需自备的设备及试剂1.450±10nm 滤光片的酶标仪 (建议仪器使用前提前预热)2.单道或多道微量加液器及吸头
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
【申请上传】Current Protocols in Immunology
and Human Interleukin 9 Unit 6.14 Measurement of Mouse and Human Interleukin 10 Unit 6.15 Measurement of Human Interleukin 11 Unit 6.16 Measurement of Human and Mouse Interleukin-12 Unit 6.17 Measurement of Human and Murine Stem Cell Factor (c-kit Ligand
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