- $590
- LSBio
- 8.192 - 800 pg/ml
- LS-F306
- 美国
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Pig
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
8.192 - 800 pg/ml
- 供应商：
康朗生物
- 规格：
96T
Pig IL8 / Interleukin 8 ELISA Kit (Sandwich ELISA)
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Product Description
LS-F306 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Pig IL8 / Interleukin 8 in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of IL8 / Interleukin 8 as low as 8.192 picograms per milliliter.Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
IL8 / Interleukin 8
Reactivity
Pig
Specificity
The antibody pair provided in this kit recognizes pig IL8.
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
8.192 - 800 pg/ml
Sensitivity
8.192 pg/ml
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Protocol
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl standard or sample to each well.
- Incubate 2.5 hours at room temperature or over night at 4°C.
- Add 100 µl prepared biotin antibody to each well.
- Incubate 1 hour at room temperature.
- Add 100 µl prepared Streptavidin solution.
- Incubate 45 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well.
- Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well.
- Read at 450 nm immediately.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About IL8 / Interleukin 8
P10145 NM_000584 NP_000575.1
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Sandwich ELISA Platform Overview
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
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