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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液体
- 保存条件:
4度
- 克隆性:
Monoclonal Antibody
- 标记物:
FITC
- 适应物种:
human
- 宿主:
Mouse
- 应用范围:
C, F, IF, P*, WB
- 浓度:
请参考说明书
- 靶点:
CYTOKERATIN 4/5/6/8/10/13/18
- 抗体英文名:
MOUSE ANTI HUMAN CYTOKERATIN 4/5/6/8/10/13/18:FITC
- 抗体名:
MOUSE ANTI HUMAN CYTOKERATIN 4/5/6/8/10/13/18:FITC
- 规格:
100 TESTS/1ml
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文献和实验Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Bromodeoxyuridine Staining of Mammary Tissue
and incubate sections overnight at 4oC with anti-BUdR-POD antibody (Boehringer Mannheim Cat.# 1 585 860) diluted 1:15 in PGB diluent. 11. Rinse sections 3 x 5 min in PBS. 12. Incubate with unactivated DAB for 5 minutes followed by activated DAB for 10 minutes
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