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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
MAGIC RED™ CATHEPSIN K KIT
- 应用范围：
IF
- 宿主：
N/A
- 标记物：
Kit
- 是否单克隆：
Kits
- 亚型：
CATHEPSIN K
- 规格：
25 TESTS
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文献和实验the secondary antibody at a 1/400 dilution. Apply 150µl and incubate 30 min., RT in a humid chamber. Blot off the antibody, wash 2 times 5 min. each in 1x PBS. Prepare the working dilution of ABC-Vector Red complex from the Vector ABC kit (Catalog No. AK
were labeled with YO-PRO-1 dye (green) (Y3603). Necrotic cells were detected with propidium iodide (red) 10 µM喜树碱诱导的Jurkat cells（慢淋。G1检测点缺陷细胞） 凋亡。细胞然后经 Vybrant Apoptosis Assay Kit #4 (V13243).处理。凋亡细胞核被YO-PRO-1绿染，坏死细胞被pi红染。 Jurkat human T-cell leukemia
and then centrifuge at 14,000 RPM for 3 minutes. Save 20µl of the elutes as (Flag-Input). Next, take the remaining ~80µl of elute to clean tubes containing 40µl of EZVIEW™ Red anti-Flag M2 affinity gel beads (Sigma™) which have been pre-treated with a wash in 250µl
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