SLIDE-A-LYZER® DIALYSIS CASSET

【资源】蛋白质浓缩，两个小tips

. It should be noted that several commercial kits for performing microdialysis are also available. For example, use of microdialysis cassettes (Slide-A-Lyzer, Pierce or Dispo-Dialyzer, Spectrum) eliminates the need for boiling or presoaking dialysis tubing

Preparation of High Titer Adenovirus in P11 cells

for 16 � 20 hr at 4° and 35K rpm.  Overlay virus with mineral oil prior to balancing as these tubes are not sealed.   -Collect the virus (I pull band with a needle at this point) in smallest volume possible, transfer to a Slide-A-Lyzer

Construction and Characterization of Adenovirus Vectors

before use. Equipment Agarose gel electrophoresis equipment Bottles (polypropylene, 500- and 1000-mL) Centrifuge (high-performance) (e.g., Avanti J-series, Beckman) Cryovials (4-mL) Dialysis cassettes (Slide-A-Lyzer, 10,000-kDa