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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
PEROXIDE BLOCKING REAGENT
- 应用范围:
P
- 宿主:
N/A
- 标记物:
Reagent
- 是否单克隆:
Accessory Reagent
- 亚型:
PEROXIDE BLOCKING REAGENT
- 规格:
50 ml
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文献和实验Lumigen HyPerBlu provides sensitive chemiluminescent detection of hydrogen peroxide
Abstract Lumigen HyPerBlu reagent is a one solution chemical substrate based on dioxetane chemiluminescent technology. With HyPerBlu reagent, a direct reaction with hydrogen peroxide produces an intense sustained luminescent signal making
Blocking of unwanted non-specific staining
and other cell types. By exploiting the preferential avidity of Fc receptor for human > mouse Ig> rabbit > swine > goat immunoglubulins, one may use a blocking of the receptors with a reagent which will not interfere with the secondary reagents
Western blotting using ECL reagent
membrane (Amersham Life Science; cat # RPN2020D)Kodak X-OMAT (XAR-5, 18X24 cm; cat # 8532665)10X TBS12.11 g Tris-base (100 mM)87.66 g NaCl (1500 mM)1 liter dd H2OAdjust pH= 7.6Washing buffer (TBS-T): 100 ml 10X TBS + 900 ml dd H2O + 1 ml Tween-20.Blocking
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