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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
多克隆抗体
- 抗体英文名：
Anti-type II collagen (CB11) antibody, clone D1-2G, biotinylated, 1 mg/ml x 0.1 ml
- 抗体名：
Anti-type II collagen (CB11) antibody, clone D1-2G, biotinylated, 1 mg/ml x 0.1 ml
- 规格：
ea
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文献和实验, change 2 x daily). 10) Centrifuge dialysed collagen at 30,000 x g for 1.5 hrs. 11) Remove supernatant and place in sterile flask. 12) Adjust the collagen I concentration to ˜2 mg/ml using 0.5 mM acetic acid at 4°C.
Bromodeoxyuridine Staining of Mammary Tissue
and incubate sections overnight at 4oC with anti-BUdR-POD antibody (Boehringer Mannheim Cat.# 1 585 860) diluted 1:15 in PGB diluent. 11. Rinse sections 3 x 5 min in PBS. 12. Incubate with unactivated DAB for 5 minutes followed by activated DAB for 10 minutes
V294.PART II，Chapter 2 Boyden法体外趋化试验
9791; Sigma-Aldrich): dissolve collagen at 1mg/mL in 0.1 N acetic acid. Allow to stir at room temperature until dissolved(takes 1–3 h). Keep collagen stock at 4°C. 9. Methanol. 10. Giemsa stain, modified solution (cat. no. GS500; Sigma-Aldrich
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