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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
多克隆抗体
- 抗体英文名：
Anti-type II collagen (CB11) antibody, clone D1-2G, 1 mg/ml x 0.1 ml
- 抗体名：
Anti-type II collagen (CB11) antibody, clone D1-2G, 1 mg/ml x 0.1 ml
- 规格：
ea
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
throughout the procedure. d) Plate the entire fusion out in just 120 x 1ml cultures in 6 x 'Linbro' 24 well plates, using DMEM + 20% FCS (containing penniclin + streptomicin, Na pyruvate and glutamine). e) DO NOT use Isoves medium (IMDM) as this will encourage
Clone Genes From a Phage Library
for phage. 2.9g NaCl(50 mM), 1g Mg SO4 (10 mM), 25 ml Tris pH 7.5 (50 mM), 50 mg gelatin (100 g/l) in 500 ml-- autoclave. • NaOH for plaque lifts: 0.5M NaOH, 1.5M Na Cl, 1 mM EDTA. • Tris for plaque lifts: 0.5M Tris HCl pH 8, 1.5 M
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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