Human IL6 / Interleukin 6 ELIS

Human IL6 / Interleukin 6 ELISA Kit (Sandwich ELISA)

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LS-F9982-1
1 plate
$640 USD
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10% off 5+ units
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Product Description

LS-F9982 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human IL6 / Interleukin 6 in samples of Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates and Urine. It is based upon a Sandwich assay principle and can be used to detect levels of IL6 / Interleukin 6 as low as 2.453 picograms per milliliter.

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Specifications

Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
IL6 / Interleukin 6
Reactivity
 
Human
Specificity
 
This assay has high sensitivity and excellent specificity for detection of human IL-6. No significant cross-reactivity or interference between human IL-6 and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human IL-6 and all the analogs, therefore, cross reaction may still exist.
Manual
 

Intended Sample Types
 
Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates, Urine
Sample Size
 
50 - 100 µl
Format
 
96-Well Microplate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
7.8 - 500 pg/ml
Sensitivity
 
2.453 pg/ml
Precision
 
Intra-assay: CV%<8% Inter-assay: CV%<10% Intra-AssayInter-Assay
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Background
 
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
About IL6 / Interleukin 6
 
Uniprot: P05231 NCBI: NM_000600 NP_000591.1

 

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