Biotin

Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and Biotin or Biotin Derivatives

Biotin-4-fluorescein (B4F) is a convenient molecular probe for (strept)avidin and for unlabeled biotin in homogeneous fluorescence assays. The primary standard is a 16 μM working solution of d-biotin which is used to titrate an aliquot

【交流】DIG/Biotin标记的Southern/Northern杂交专题讨论

Southern印迹杂交（Southern blot）是1975年由英国人southern创建，是研究DNA图谱的基本技术，在遗传病诊断、DNA图谱分析及PCR产物分析等方面有重要价值。 早期的Southern印迹是将凝胶中的DNA变性后，经毛细管的虹吸作用，转移到硝酸纤维膜上。印迹方法如电转法、真空转移法；滤膜发展了尼龙膜、化学活化膜（如APT、ABM纤维素膜）等。利用Southern印迹法可进行克隆基因的酶切、图谱分析、基因组中某一基因的定性及定量分析、基因突变分析及限制性片断长度多态

Conjugation of Antibodies to Biotin

Antibodies can be conjugated to biotin by a number of chemical means. They can then be used in immunochemical procedures in conjunction with secondary reagents coupled to biotin-binding protein proteins such as avidin.