小鼠 ECM1蛋白|ECM1 protein|ECM1(Mouse, C-His)(产品说明)
蛋白名称:ECM1蛋白, ECM1 protein
蛋白构建:A DNA sequence encoding the mouse ECM1 isoform 1 (Q61508-1) (Met 1-Glu 559) was expressed, fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 88 % as determined by SDS-PAGE
蛋白活性:Measured by the ability of the immobilized protein to support the adhesion of HFF Human skin fibroblast cells. When 5 × 10E4 cells/well are added to mECM1-His coated plates (2.5 μg/mL and 100 μL/well), approximately > 45 % will adhere specifically after 90 minutes at 37 ℃.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ala 20
蛋白分子量:The secreted recombinant mouse ECM1 comprises 551 amino acids and has a predicted molecular mass of 62.5 kDa. As a result of glycosylation, the apparent molecular mass of rm ECM1 is approximately 90-95 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:Q61508-1
蛋白氨基酸序列:Met1-Glu559
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.