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- Sino Biological
- 90043-K02H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
100 µg
蛋白名称：HER3/ERBB3蛋白, HER3/ERBB3 protein
蛋白构建：A DNA sequence encoding the Rhesus (Macaca mulatta) ErbB3 (XP_001113953.2) extracellular domain (Met 1-Thr 643) was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：1. Measured by its ability to human NRG1 (aa 20-241) (Cat:13499-H02H) in functional Elisa. 2. Measured by its ability to human NRG1 (aa 177-241) (Cat:13499-H01H) in functional Elisa. 3. Measured by its ability to human NRG1 (aa 20-241)-His (Cat:13499-H08H) in functional Elisa.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ser 20
蛋白分子量：The recombinant Rhesus ErbB3/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 865 amino acids and predicts a molecular mass of 95.7 kDa. As a result of glycosylation, rhesus ErbB3/Fc monomer migrates as an approximately 130-140 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：XP_001113953.2
蛋白氨基酸序列：Met1-Thr643
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验注意的是，两种配体的洗脱和再生buffer稍有差别，使用的时候还需注意！ 系统特点 高达 pM 范围的亲和力(KD up to 6.2 x 10-11 M) 纯化树脂材质稳定 小标签 (8-28 aa)无需移除，不影响蛋白折叠和功能 极高的蛋白纯度（纯度＞95%） 应用于多种目标蛋白检测系统：Western blot, ELISA, 免疫荧光, FACS等 兼容多种缓冲条件：高盐，洗涤剂，金属离子，螯合剂，还原剂 适用于多种蛋白的纯化 Twin-Strep-tag®标签蛋白固定在界面上时 (SPR
receptor) signaling pathway. The pathway consists of at least four Cell ular receptors: EGFR/HER1, HER2, HER3, and HER4. Approximately 11 different factors are known to bind and activate these receptors in certain patterns. The HER signaling pathway plays
CUT&Tag（Cleavage Under Targets and Tagmentation）【1】是一种研究蛋白质-DNA 相互作用的新技术。与传统的染色质免疫沉淀（Chromatin Immunoprecipitation, ChIP）相比，CUT&Tag 具有信噪比高、可重复性好、实验周期短（1 天实现从细胞到文库构建）、细胞投入量低等显著优势。该方法一经问世，便受到广大科研工作者的青睐。 在过去的一年时间里，科研工作者在动物、植物细胞中成功应用 CUT&Tag
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