快速mFluor 700抗体标记试剂盒，红色

AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Red 700 dyes have fluorescence excitation and emission maxima of ~657 nm and ~700 nm respectively. Like Alexa Fluor 700-APC tandem, it has strong absorption at 633 nm, making its conjugates readily excitable by 633 nm red laser. These spectral characteristics make mFluor™ Red 700 an excellent alternative to Alexa Fluor 700-APC tandem. mFluor™ Red 700 is a small organic molecule that is much easier to use than the Alexa Fluor 700-APC tandem. mFluor™ Red 700 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. This kit has all the essential components to perform two separate labeling reactions with no column purification needed. Each of the two vials of mFluor™ Red 700 provided in the kit is optimized for labeling ~50 µg antibody. mFluor™ Red 700 antibody labeling kit provides a convenient method to label small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the mFluor™ Red 700 dye.

Components

Component A: mFluor™ Red 700	2 vials (One vial is for 50 μg protein)
Component B: Reaction Buffer	1 vial (20 μL)
Component C: TQ™-Dyed Quench Buffer	1 vial (20 μL)

Example protocol