- $1220
- LSBio
- 0.312 - 20 ng/ml
- LS-F51247
- 美国
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Bovine
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
0.312 - 20 ng/ml
- 供应商：
康朗生物
- 规格：
96T
Bovine IL2RA / CD25 ELISA Kit (Sandwich ELISA)
Product Description
LS-F51247 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Bovine IL2RA / CD25 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of IL2RA / CD25 as low as 0.078 nanograms per millilter.Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
IL2RA / CD25
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of bovine IL2RA. No significant cross-reactivity or interference between bovine IL2RA and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between bovine IL2RA and all the analogues, therefore, cross reaction may still exist.
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Sample Size
50 - 100 µl
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.312 - 20 ng/ml
Sensitivity
0.078 ng/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<8% / Inter-Assay: CV<10%
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About IL2RA / CD25
P01589 NM_000417 NP_000408.1
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文献和实验ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
apparatus. Aspirate with pasteur pipets as before. A triple wash is required, this time using the Nunc washer to fill and aspirate each row 3x simultaneously before moving on to the next row. Dry the wells by removing the remaining liquid with pasteur pipets
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