Papain is a cysteine protease, found in Carica papaya. It is composed of a single polypeptide chain containing three disulfide bridges and a sulfhydryl group.
Application
Papain from papaya latex has been used: • to digest carotid artery smooth muscle cells • in dissecting solutions • to produce Fab fragments of antibodies • for cell dissociation since it has been shown to be more effective and less damaging with certain tissues • in the enzyme cocktail for dissociating human fetal retinal tissue • to digest chondrocytes/scaffold cultures for total DNA quantification for cell proliferation • as a supplement in neurobasal medium to produce retinal cell suspensions