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- 普利莱
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- 2025年12月20日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期：
1年
- 库存：
大量
- 英文名：
RIPA Lysis Buffer
- 供应商：
普利莱
- 保存条件：
4℃
- 规格：
100ml/500ml
| 规格： | 100ml | 产品价格： | ￥130.0 |
|---|---|---|---|
| 规格： | 500ml | 产品价格： | ￥550.0 |
描述：RIPA裂解液(RIPA Lysis Buffer)对动物细胞胞膜、胞浆、胞核成分均有较强裂解作用，是经典和最常用的细胞组织快速裂解液。使用RIPA 裂解缓冲液制备用于Western特别是用于免疫共沉淀的裂解产物已经是一种标准操作，也适用于大部分抗原表位检测，特别是免疫共沉淀等实验。
使用我司RIPA裂解缓冲液发表SCI论文已达数百篇，部分文章列表如下，供参考：
1、Corporation H P. Secernin-1 Contributes to Colon Cancer Progression through Enhancing Matrix Metalloproteinase-2/9 Exocytosis.[J]. Disease Markers, 2015.
2、Corporation H P. Cytotoxic Effects of Biosynthesized Zinc Oxide Nanoparticles on Murine Cell Lines[J]. Evidence-based Complementary and Alternative Medicine : ECAM, 2015, 2015.
3、Corporation H P. Protective effects of N-acetylcysteine in concanavalin a-induced hepatitis in mice.[J]. Mediators Inflamm, 2015.
4、Corporation H P. Blockade of lysosomal acid ceramidase induces GluN2B-dependent Tau phosphorylation in rat hippocampal slices.[J]. Neural Plasticity, 2014,
5、J. V, T. P, Y. L, et al. Androgen Receptor Activation[J]. Cellular Physiology & Biochemistry, 2014, 33(6):1911-1920.
6、Yang K, Song Y, Tang Y, et al. mAChRs activation induces epithelial-mesenchymal transition on lung epithelial cells.[J]. Bmc Pulmonary Medicine, 2014, 14(4):417-419.
7、Peng Y, Guo J, Liu Y, et al. MicroRNA-34A inhibits the growth, invasion and metastasis of gastric cancer by targeting PDGFR and MET expression[J]. Bioscience Reports, 2014, 34(2):247-256.
8、Lee S Y. Nonthermal plasma induces apoptosis in ATC cells: involvement of JNK and p38 MAPK-dependent ROS.[J]. Yonsei Medical Journal, 2014, 55(6).
9、Jeong J, Conboy M J, Conboy I M. Pharmacological inhibition of myostatin/TGF-β receptor/pSmad3 signaling rescues muscle regenerative responses in mouse model of type 1 diabetes[J]. Acta Pharmacologica Sinica, 2013, 34:1052-1060.
10、Vaziri N D, Yuan J, Nazertehrani S, et al. Chronic Kidney Disease Causes Disruption of Gastric and Small Intestinal Epithelial Tight Junction.[J]. American Journal of Nephrology, 2013, 38(2):99-103.
11、Li Y, Sun Y, Liu F, et al. Norcantharidin Inhibits Renal Interstitial Fibrosis by Blocking the Tubular Epithelial-Mesenchymal Transition[J]. Plos One, 2013, 8(6):e66356.
12、Kashyap M, Kawamorita N, Tyagi V, et al. Down-regulation of nerve growth factor expression in the bladder by antisense oligonucleotides as new treatment for overactive bladder.[J]. Journal of Urology, 2013, 190(2):757-764.
13、Li T, Kilic A, Wei X, et al. Regional imbalanced activation of the calcineurin/BAD apoptotic pathway and the PI3K/Akt survival pathway after myocardial infarction.[J]. International Journal of Cardiology, 2013, 166(1):158–165.
使用我司RIPA裂解缓冲液发表SCI论文已达数百篇，部分文章列表如下，供参考：
1、Corporation H P. Secernin-1 Contributes to Colon Cancer Progression through Enhancing Matrix Metalloproteinase-2/9 Exocytosis.[J]. Disease Markers, 2015.
2、Corporation H P. Cytotoxic Effects of Biosynthesized Zinc Oxide Nanoparticles on Murine Cell Lines[J]. Evidence-based Complementary and Alternative Medicine : ECAM, 2015, 2015.
3、Corporation H P. Protective effects of N-acetylcysteine in concanavalin a-induced hepatitis in mice.[J]. Mediators Inflamm, 2015.
4、Corporation H P. Blockade of lysosomal acid ceramidase induces GluN2B-dependent Tau phosphorylation in rat hippocampal slices.[J]. Neural Plasticity, 2014,
5、J. V, T. P, Y. L, et al. Androgen Receptor Activation[J]. Cellular Physiology & Biochemistry, 2014, 33(6):1911-1920.
6、Yang K, Song Y, Tang Y, et al. mAChRs activation induces epithelial-mesenchymal transition on lung epithelial cells.[J]. Bmc Pulmonary Medicine, 2014, 14(4):417-419.
7、Peng Y, Guo J, Liu Y, et al. MicroRNA-34A inhibits the growth, invasion and metastasis of gastric cancer by targeting PDGFR and MET expression[J]. Bioscience Reports, 2014, 34(2):247-256.
8、Lee S Y. Nonthermal plasma induces apoptosis in ATC cells: involvement of JNK and p38 MAPK-dependent ROS.[J]. Yonsei Medical Journal, 2014, 55(6).
9、Jeong J, Conboy M J, Conboy I M. Pharmacological inhibition of myostatin/TGF-β receptor/pSmad3 signaling rescues muscle regenerative responses in mouse model of type 1 diabetes[J]. Acta Pharmacologica Sinica, 2013, 34:1052-1060.
10、Vaziri N D, Yuan J, Nazertehrani S, et al. Chronic Kidney Disease Causes Disruption of Gastric and Small Intestinal Epithelial Tight Junction.[J]. American Journal of Nephrology, 2013, 38(2):99-103.
11、Li Y, Sun Y, Liu F, et al. Norcantharidin Inhibits Renal Interstitial Fibrosis by Blocking the Tubular Epithelial-Mesenchymal Transition[J]. Plos One, 2013, 8(6):e66356.
12、Kashyap M, Kawamorita N, Tyagi V, et al. Down-regulation of nerve growth factor expression in the bladder by antisense oligonucleotides as new treatment for overactive bladder.[J]. Journal of Urology, 2013, 190(2):757-764.
13、Li T, Kilic A, Wei X, et al. Regional imbalanced activation of the calcineurin/BAD apoptotic pathway and the PI3K/Akt survival pathway after myocardial infarction.[J]. International Journal of Cardiology, 2013, 166(1):158–165.
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文献和实验该产品被引用文献
- Liu X, Chen C, Han D, et al. SLC7A11/GPX4 inactivation-mediated ferroptosis contributes to the pathogenesis of triptolide-induced cardiotoxicity[J]. Oxidative Medicine and Cellular Longevity, 2022, 2022.
- Wu F, Cao W, Xu H, et al. Treatment with a selenium-platinum compound induced T-cell acute lymphoblastic leukemia/lymphoma cells apoptosis through the mitochondrial signaling pathway[J]. Oncology Letters, 2017, 13(3): 1702-1710.
- Xiang S, Zhu L, Zhang Z, et al. Proteomic analysis of inhibitor of apoptosis proteinlike protein2 on breast cancer cell proliferation[J]. Molecular Medicine Reports, 2022, 25(3): 1-11.
- Gao C, Liu S G, Lu W T, et al. Downregulating CREBBP inhibits proliferation and cell cycle progression and induces daunorubicin resistance in leukemia cells[J]. Molecular Medicine Reports, 2020, 22(4): 2905-2915.
- Zhou W, Yuan X, Zhang L, et al. Overexpression of HO-1 assisted PM2. 5-induced apoptosis failure and autophagy-related cell necrosis[J]. Ecotoxicology and environmental safety, 2017, 145: 605-614.
- Yu Z, Chen N, Hu D, et al. Decreased density of perineuronal net in prelimbic cortex is linked to depressive-like behavior in young-aged rats[J]. Frontiers in molecular neuroscience, 2020, 13: 4.P1265P1511
- Jiang Q H, Wang A X, Chen Y. Radixin enhances colon cancer cell invasion by increasing MMP-7 production via Rac1-ERK pathway[J]. The Scientific World Journal, 2014, 2014.P1265
- Yin L, Chen X, Li N, et al. Puerarin ameliorates skeletal muscle wasting and fiber type transformation in STZ-induced type 1 diabetic rats[J]. Biomedicine & Pharmacotherapy, 2021, 133: 110977.P1010B1012
- Shen T, Xu F, Fang Z, et al. Hepatic autophagy and mitophagy status in dairy cows with subclinical and clinical ketosis[J]. Journal of Dairy Science, 2021, 104(4): 4847-4857.
- Gu Y, Jin J, Zhang S. GW26-e4374 Tanshinone IIA Protects H9c2 Cells From Oxidative Stress-Induced Cell Death Via Up-Regulation of MicroRNA-133 and Akt Activation[J]. Journal of the American College of Cardiology, 2015, 66(16S): C36-C37.
相关实验
[3] 将细胞裂解物分离成 RIPA 可溶性和 RIPA 不可溶性组分，发现在两个组分中都可以检测到 TDP-43 的磷酸化片段。同样的，用 RIPA 缓冲液裂解小鼠 MEC 细胞时，磷酸化的 EGFR（pY-1068 EGFR）只在 RIPA 可溶性组分中检测出。然而，磷酸化的 c-Src（pY-416 c-Src）则在 RIPA 可溶性和 RIPA 不可溶组分中都能检测出， 甚至 WT 样品在 RIPA 不可溶组分中的信号比 RIPA 可溶性组分中更强（下图），结果表明如果仅使用 RIPA 可溶性组分
样本准备 蛋白提取 所需仪器：匀浆器、RIPA 裂解液、PMSF（蛋白酶抑制剂）、磷酸酶抑制剂、剪刀、镊子，消毒酒精棉、PBS、移液器、离心机。 裂解液配制——RIPA 裂解液：PMSF=100：1。 1） 组织处理：首先用酒精棉消毒剪刀和镊子，取适量的组织于匀浆器中，用剪刀剪碎加适量的裂解液冰水中匀浆 1-4 min。匀浆结束后吸取溶液于 EP 管中保存备用。（含血液较多的组织可先用 PBS 清洗后再匀浆） 2） 细胞处理： A、 贴壁细胞处理：去掉培养基用 PBS 清洗后加入适量的裂解
多篇 SCI 发现用 RIPA 提蛋白存在问题，我们该如何应对？
90, c-Src, 和 tubulin，表明这些蛋白在 RIPA 不可溶组分中大量存在（下图）。Wang 等 [6] 对比 SDS 加热法和 RIPA 裂解液法从斑马鱼肝脏肿瘤中提取蛋白，发现 RIPA 裂解液提取这些样品时对于大分子量蛋白的提取效率十分低（下图）。Li[7] 对比了从小鼠脾脏和肝脏中提取总蛋白 RIPA 可溶和不可溶性组分以及基于离心管柱法商业试剂盒提取的蛋白质图谱，发现 RIPA 不可溶组分蛋白质图谱与可溶性组分图谱相似，但是不完全相同。这些丢失的不可溶组分覆盖了整个蛋白质图谱分子
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RIPA裂解缓冲液 C1053 厂家直销，提供OEM定制服务，大包装更优惠
￥130 - 550
