CAL 27 人舌鳞癌细胞

CAL 27 人舌鳞癌细胞

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  • 2025年11月18日
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    • 细胞类型：

      普通细胞株-科研实验

    • 物种来源：

      人

    • 运输方式：

      常温或干冰

    • 生长状态：

      良好

    • 供应商：

      复祥生物

    • 库存：

      大量

    CAL 27 人舌鳞癌细胞

    DMEM+10%胎牛血清。细胞货期8-10个工作日

    上海复祥生物提供 ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和最优培养条件,网站上有细胞照片,欢迎各位老师来电咨询!联系电话400-821-8510 手机15000266580
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    ATCC® Number: CRL-2095™ Price:
    Designations: CAL 27
    Depositors: C Cardona
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens (human)
    Morphology: epithelial
    Source: Organ: tongue
    Disease: squamous cell carcinoma
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Isolation: Isolation date: 1982
    Tumorigenic: Yes
    DNA Profile (STR): Amelogenin: X
    CSF1PO: 10,12
    D13S317: 10,11
    D16S539: 11,12
    D5S818: 11,12
    D7S820: 10
    THO1: 6,9.3
    TPOX: 8
    vWA: 14,17
    Cytogenetic Analysis: aneuploid; modal number = 43
    Age: 56 years
    Gender: male
    Ethnicity: Caucasian
    Comments: Cal 27 was established in 1982 by J. Gioanni (Centre Antoine Lacassagne, Nice Cedex, France) from tissue taken prior to treatment from a 56 year old Caucasian male with a lesion of the middle of the tongue.
    CAL 27 cells are epithelial, polygonal with a highly granular cytoplasm.
    Immunocytochemical studies show strong positive staining with anti keratin antibodies.
    The cells do not grow well in semi-solid medium.
    Marked inhibition of thymidine incorporation was observed in the presence of VP16 (etoposide), CCNU (1-[2-chloroethyl]-3-cyclohexyl-1-nitrosourea), VM26 (teniposide), ADM (adriamycin), CPA (cyclophosphamide), and MTX (methotrexate).
    CAL 27 cells were resistant to treatment with VDS (vindesine sulfate), CDP (cis-platinum) or ACTD (actinomycin D).
    A culture submitted to the ATCC in December 1993 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0°C
    Subculturing: Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove trypsin. Add fresh trypsin and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
    Subcultivation Ratio: A subcultivation ratio of 1:6 is recommended
    Medium Renewal: Every 2 to 3 days
    Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor temperature
    Doubling Time: 35 hrs
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
    recommended serum:ATCC 30-2020
    References: 23521: Gioanni J, et al. Two new human tumor cell lines derived from squamous cell carcinomas of the tongue: establishment, characterization and response to cytotoxic treatment. Eur. J. Cancer Clin. Oncol. 24: 1445-1455, 1988. PubMed: 3181269

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