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- 详细信息
- 文献和实验
- 技术资料
- 库存:
357
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 规格:
10μg
特别提示:包括M13mp18 RF I DNA在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:M13mp18 RF I DNA
产品货号:SV1183
产品规格:10μg
特性:
• 噬菌体 M13 衍生噬菌体载体
• 共价闭环 DNA
• β-半乳糖甘酶 13 个独特的 RE 位点
• 蓝/白斑筛选
浓度:
100 μg/ml
分子量/长度
7,249 bp
除了M13mp18 RF I DNA,,我公司还供应以下相关产品:
SV0823 稀释液 C 5ml
SV1192 β-琼脂糖酶 I 500U|100U
SV1275 Quick-Load 低分子量 DNA Ladder 125gel lanes
SV1306 pBR322 DNA-Msp I 消化 250gel lanes|50gel lanes
SV1324 彩色预染蛋白 Standard,宽范围 (11–245 kDa) 500gel lanes|100gel lanes|50gel lanes
SV1400 polyA Spin mRNA 分离试剂盒 8次分离反应
SV1585 McrBC限制性内切酶 2500U|500U
SV1626 CLIP-Cell TMR-Star 30 nmol
SV1632 SNAP-Surface Alexa Fluor 546 50 nmol
SV1635 SNAP-Cell 647-SiR 30 nmol
SV1652 pCMV-CLuc 2 对照质粒 20μg
SV1661 pGLuc-Basic 2 载体 20μg
ZN1533 *高辛随机引物DNA标记复合物 25μl
ZN1534 寡核苷酸探针稀释液 1ml
ZN1536 探针稀释液(cDNA) 1ml
QN0544 酵母tRNA 100mg
QN0572 四氧嘧啶 5g
BTN160118 0.2M乙酸缓冲液(pH5.6) 250mL
BTN160134 0.2M硼*缓冲液(pH7.8) 250mL
BTN160103 Michel缓冲液 250mL
BTN160124 0.1M磷酸缓冲液(pH6.0) 250mL
BTN160108 Sorensen磷酸缓冲液(0.2M,pH7.4) 250mL
MT0105 红细胞裂解液 500ml
GL1340 即用型细菌冻存液 50ml
GL1395 Tris-HCl缓冲液(1mol/L,pH6.8) 100ml|500ml
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文献和实验Cloning DNA Fragments in M13 Vectors
The genome of the filamentous bacteriophage M13 comprises a single-stranded circular DNA molecule, designated the (+) strand, which is converted into a double-stranded replicative form (RF) on infection of Escherichia coli . Cloning vectors based
Transfection of E. coli with M13 DNA
In recent years several techniques have been described for the introduction of DNA molecules into strains of E. coli by transformation or transfection. These are based on the findings of Mandel and Higa (1 ) who demonstrated that incubation
Reverse Sequencing of M13 Cloned DNA
When shotgun sequencing projects are almost complete, continued random sequencing becomes increasingly less fruitful. The sequence may have regions of data that have only been determined on one strand (and therefore potentially error prone
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