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文献和实验, Type Culture Collection of Chinese Academy of Sciences) were cultured in RPMI1640 supplemented with 10% heat-inactivated FBS, 100 U/ml penicillin, 100 mg/ml streptomycin, and 2 mM glutamine. Human embryonic kidney (HEK) 293 cells (American Type Culture
Q:We recently completed a ChIP-seq run on the ABI SOLiD. We received the following data back for one of our samples. These data were from a quarter (1/4) of a slide. ------------------61993194 total beads foundTotal Beads0 mismatches 5631945 ( 9.08
SOLID TUMOR CULTURES FOR CHROMOSOME ANALYSIS
so that it layers on top of the cell suspension. Mix rapidly by hand so that all the cell suspension is fixed evenly. Wash down sides of tube with another pipetful of fix. Let sit at room temperature for 15 - 20 minutes. G. Centrifuge for 6 minutes at 150 x g
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