Sigma-Aldrich-Millipore货号MABN2

Arrestin-C (UniProt: A0A2K5U9A7; also known as Cone arrestin, Retinal cone arrestin-3) is encoded by the ARR3 gene (Gene ID: 102134564) in Monkey. Arrestins are a superfamily of multi-functional proteins that that regulate signaling and trafficking of the majority of G-protein-coupled receptors (GPCRs), as well as sub-cellular localization and activity of many other signaling proteins. Arrestin-C is predominantly found in inner and outer segments, and the inner plexiform regions of the retina. It is expressed in cone photoreceptors and pinealocytes and may contribute to the shut-off mechanisms associated with high acuity color vision. Arrestin-C is an elongated two-domain molecule with overall fold and key inter-domain interactions that hold the free protein in the basal conformation similar to the other subtypes. Several structural elements are reported to contribute to arrestin binding. The C-terminal acidic region serves a regulatory role in controlling arrestin binding selectivity toward the phosphorylated and activated form of a receptor. The basic N-terminal domain directly participates in receptor interaction and serves a regulatory role via intramolecular interaction with the C-terminal acidic region. Also, two centrally localized domains are directly involved in determining receptor binding specificity and selectivity. Clone 7G6 is a cone-specific marker that recognizes all cones in the adult primate retina. In fetal retina, although long and middle wavelength-sensitive cones recognized by clone 7G6, a subset of short wavelength-sensitive cones are shown to be delayed in their reactivity to this clone. (Ref.: Zhang, H., et al. (2003). Invest. Opthalmol. Vis. Sci. 44(7); 2858-2867; Wikler, KC., et al. (1997). J. Comp. Neurol. 377(4); 500-508).

Quality Control Testing

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.

Tested Applications

Western Blotting Analysis: A representative lot detected Arrestin-C in Western Blotting applications (Zhang, H., et al. (2003). Invest Ophthalmol Vis Sci.;44(7):2858-67).

Immunohistochemistry Applications: A representative lot detected Arrestin-C in Immunohistochemistry applications (Wikler, K.C., et al. (1997). J Comp Neurol.;377(4):500-8; John, S.K., et al. (2000). Mol Vis.;6:204-15; Zhang, H., et al. (2003). Invest Ophthalmol Vis Sci.;44(7):2858-67; O′Brien, J.J., et al. (2012). J Neurosci.;32(13):4675-87).

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Arrestin-C in frozen monkey retina tissues (Courtesy of Prof. Peter MacLeish, Ph.D., photo by Talib Saafir, Ph.D., Morehouse School of Medicine, Atlanta, GA, USA).

Immunofluorescence Analysis: A representative lot detected Arrestin-C in Immunofluorescence applications (Zhang, H., et al. (2003). Invest Ophthalmol Vis Sci.;44(7):2858-67).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Arrestin-C, clone 7G6, Cat. No. MABN2636, is a mouse monoclonal antibody that detects Arrestin-C and is tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.